ArticleThe protein journal2006
Botulinum neurotoxin light chain refolds at endosomal pH for its translocation.
Article in The protein journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed.
- A DARPin promotes faster onset of botulinum neurotoxin A1 action.Nature communications · 2023Article
- A Comprehensive Structural Analysis ofToxins · 2023Review
- In Silico Conformational Features of Botulinum Toxins A1 and E1 According to Intraluminal Acidification.Toxins · 2022Article
- Clostridial Neurotoxins: Structure, Function and Implications to Other Bacterial Toxins.Microorganisms · 2021Review
- Camelid VHH Antibodies that Neutralize Botulinum Neurotoxin Serotype E Intoxication or Protease Function.Toxins · 2020Article
- A novel role of C-terminus in introducing a functionally flexible structure critical for the biological activity of botulinum neurotoxin.Scientific reports · 2018Article
- Article
- Structural and functional analysis of botulinum neurotoxin subunits for pH-dependent membrane channel formation and translocation.Biochimica et biophysica acta · 2015Article
- The Effect of pH on Globular State of Lipase-3646; an Appropriate Model for Molten Globule Investigations.The protein journal · 2015Article
- A Heterologous Reporter Defines the Role of the Tetanus Toxin Interchain Disulfide in Light-Chain Translocation.Infection and immunity · 2015Article
- Systematic discovery of molecular probes targeting multiple non-orthosteric and spatially distinct sites in the botulinum neurotoxin subtype A (BoNT/A).Molecular and cellular probes · 2015Article
- Tetanus neurotoxin utilizes two sequential membrane interactions for channel formation.The Journal of biological chemistry · 2014Article
- Effects of enzymatically inactive recombinant botulinum neurotoxin type A at the mouse neuromuscular junctions.Toxicon : official journal of the International Society on Toxinology · 2013Article
- Development of a fluorescence internal quenching correction factor to correct botulinum neurotoxin type A endopeptidase kinetics using SNAPtide.Analytical chemistry · 2012Article
- The identification and biochemical characterization of drug-like compounds that inhibit botulinum neurotoxin serotype A endopeptidase activity.Toxicon : official journal of the International Society on Toxinology · 2010Article
- Botulinum neurotoxin devoid of receptor binding domain translocates active protease.PLoS pathogens · 2008Article
- Single molecule detection of intermediates during botulinum neurotoxin translocation across membranes.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Comparative membrane channel size and activity of botulinum neurotoxins A and E.The protein journal · 2007Article
- Lipid and cationic polymer based transduction of botulinum holotoxin, or toxin protease alone, extends the target cell range and improves the efficiency of intoxication.Toxicon : official journal of the International Society on ToxinologyArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Botulinum neurotoxins (BoNTs), the most poisonous member of class A biothreat agent, cause neuroparalysis by blocking neurotransmitter release at the neuromuscular junctions. In its mechanism of action, the catalytic domain (light chain (LC) of BoNT) is transported to the cytosol by the heavy chain (HC) in order to reach its proteolytic substrates. The BoNT HC forms a membrane channel under acidic conditions encountered in endosomes to serve as a passageway for LC to enter into cytosol. We demonstrate here that BoNT/A LC undergoes unique structural changes under the low pH conditions, and adopts a molten globule state, exposing substantial number of hydrophobic groups. The flexibility of the molten globular structure combined with retention of the secondary structure and exposure of specific residues of LC for interaction with the HC, allows its translocation through the narrow endosomal membrane channel.
Indexed as
Identifiers
17115255What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.