ArticleInternational journal of legal medicine2007
Successful RNA extraction from various human postmortem tissues.
Article in International journal of legal medicine, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 103 citations in OpenAlex.
- Postmortem interval estimation through skeletal muscle protein degradation: a comprehensive review.International journal of legal medicine · 2026Review
- Exploring the human brain: spatial transcriptomics challenges and approaches in post-mortem analysis.Brain : a journal of neurology · 2026Review
- Postmortem Changes in mRNA Expression and Tissue Morphology in Brain and Femoral Muscle Tissues of Rat.International journal of molecular sciences · 2025Article
- An experimental study to estimate the early postmortem interval based on the degradation of lncRNAs in rat brain tissue.Scientific reports · 2024Article
- Review of the current and potential use of biological and molecular methods for the estimation of the postmortem interval in animals and humans.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Review
- The ex vivo human translaminar autonomous system to study spaceflight associated neuro-ocular syndrome pathogenesis.NPJ microgravity · 2022Article
- Common Ground between Biological Rhythms and Forensics.Biology · 2022Review
- Article
- Yield and Integrity of RNA from Brain Samples are Largely Unaffected by Pre-analytical Procedures.Neurochemical research · 2021Article
- MicroRNAs as Useful Tools to Estimate Time Since Death. A Systematic Review of Current Literature.Diagnostics (Basel, Switzerland) · 2021Review
- Postmortem interval determination using mRNA markers and DNA normalization.International journal of legal medicine · 2020Article
- Tissue- and Species-Specific Patterns of RNA metabolism in Post-Mortem Mammalian Retina and Retinal Pigment Epithelium.Scientific reports · 2019Article
- A comparison of mechanistic signaling pathway activity analysis methods.Briefings in bioinformatics · 2019Review
- Effect of Postmortem Interval and Years in Storage on RNA Quality of Tissue at a Repository of the NIH NeuroBioBank.Biopreservation and biobanking · 2018Article
- The effects of death and post-mortem cold ischemia on human tissue transcriptomes.Nature communications · 2018Article
- Article
- Cell Death-Associated Ribosomal RNA Cleavage in Postmortem Tissues and Its Forensic Applications.Molecules and cells · 2017Article
- Tracing the dynamics of gene transcripts after organismal death.Open biology · 2017Article
- DNA and RNA profiling of excavated human remains with varying postmortem intervals.International journal of legal medicine · 2016Article
- Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles.Histochemistry and cell biology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, several authors described the observation that RNA degradation does not correlate with the postmortem interval (PMI), but rather with other parameters like environmental impact and the circumstances of death. Therefore, the question arose if the analysis of gene expression could be a valuable tool in forensic genetics to contribute to the determination of the cause of death. In our study, six human tissues obtained from six individuals with PMI varying between 15 and 118 h were used for total RNA extraction. Quantification was performed using a GAPDH real-time assay, and the quality of mRNA was checked by amplification of different fragment lengths of the GAPDH transcript. In our set of samples, nearly all tissues in all PMI revealed satisfactory results, while skeletal muscle, followed by brain and heart, gave the best results. No correlation between PMI and RNA degradation could be detected, as very good results were observed for all tissues from the individual with the longest PMI. The highly promising results obtained in this study raise hopes that in the near future several fields of forensic investigation may profit from additional information about gene expression patterns and their correlation with pathological findings.
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