ArticleEuropean biophysics journal : EBJ2007
Changes induced by hyperosmotic mannitol in cerebral endothelial cells: an atomic force microscopic study.
Article in European biophysics journal : EBJ, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 34 citations in OpenAlex.
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- Effects of Astragalus Polysaccharide on Mechanical Characterization of Liver Sinusoidal Endothelial Cells by Atomic Force Microscopy at Nanoscale.Chinese journal of integrative medicine · 2018Article
- Rho-Kinase Inhibition Ameliorates Dasatinib-Induced Endothelial Dysfunction and Pulmonary Hypertension.Frontiers in physiology · 2018Article
- Double-stranded RNA attenuates the barrier function of human pulmonary artery endothelial cells.PloS one · 2014Article
- Constant or fluctuating hyperglycemias increases cytomembrane stiffness of human umbilical vein endothelial cells in culture: roles of cytoskeletal rearrangement and nitric oxide synthesis.BMC cell biology · 2013Article
- Adhesion and stress relaxation forces between melanoma and cerebral endothelial cells.European biophysics journal : EBJ · 2012Article
- Casein aggregates built step-by-step on charged polyelectrolyte film surfaces are calcium phosphate-cemented.The Journal of biological chemistry · 2010Article
- Neuropathological studies of chickens following exposure to chlorpyrifos.Toxicology international · 2010Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the reaction of living cells in response to different extracellular stimuli, such as hyperosmotic stress, is of primordial importance. Mannitol, a cell-impermeable non-toxic alcohol, has been used successfully for reversible opening of the blood-brain barrier in hyperosmotic concentrations. In this study we analyzed the effect of hyperosmotic mannitol on the shape and surface structure of living cerebral endothelial cells by atomic force microscope imaging technique. Addition of clinically relevant concentrations of mannitol to the culture medium of the confluent cells induced a decrease of about 40% in the observed height of the cells. This change was consistent both at the nuclear and peripheral region of the cells. After mannitol treatment even a close examination of the contact surface between the cells did not reveal gap between them. We could observe the appearance of surface protrusions of about 100 nm. By force measurements the elasticity of the cells were estimated. While the Young's modulus of the control cells appeared to be 8.04 +/- 0.12 kPa, for the mannitol-treated cells it decreased to an estimated value of 0.93 +/- 0.04 kPa which points to large structural changes inside the cell.
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