ArticleBritish journal of pharmacology2007
Regulation of OX1 orexin/hypocretin receptor-coupling to phospholipase C by Ca2+ influx.
Article in British journal of pharmacology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Orexin Signaling: A Complex, Multifaceted Process.Frontiers in cellular neuroscience · 2022Review
- Orexin receptors in GtoPdb v.2021.3.IUPHAR/BPS guide to pharmacology CITE · 2021Article
- Orexin-A Exerts Neuroprotective Effects via OX1R in Parkinson's Disease.Frontiers in neuroscience · 2018Article
- Global analysis of gene expression mediated by OX1 orexin receptor signaling in a hypothalamic cell line.PloS one · 2017Article
- Effect of Orexin-A on Cortisol Secretion in H295R Cells via p70S6K/4EBP1 Signaling Pathway.International journal of endocrinology · 2015Article
- Lateral hypothalamic orexin and melanin-concentrating hormone neurons provide direct input to gonadotropin-releasing hormone neurons in the human.Frontiers in cellular neuroscience · 2015Article
- Calcium affects OX1 orexin (hypocretin) receptor responses by modifying both orexin binding and the signal transduction machinery.British journal of pharmacology · 2014Article
- Orexin/hypocretin based pharmacotherapies for the treatment of addiction: DORA or SORA?CNS drugs · 2014Review
- Orexins protect neuronal cell cultures against hypoxic stress: an involvement of Akt signaling.Journal of molecular neuroscience : MN · 2014Article
- Orexin/hypocretin receptor signalling: a functional perspective.British journal of pharmacology · 2014Article
- Orexin/hypocretin receptor signalling cascades.British journal of pharmacology · 2014Review
- Orexin-A promotes cell migration in cultured rat astrocytes via Ca2+-dependent PKCα and ERK1/2 signals.PloS one · 2014Article
- Role of orexin receptors in obesity: from cellular to behavioral evidence.International journal of obesity (2005) · 2013Review
- OX2R activation induces PKC-mediated ERK and CREB phosphorylation.Experimental cell research · 2012Article
- Brain orexin promotes obesity resistance.Annals of the New York Academy of Sciences · 2012Review
- OX1 orexin/hypocretin receptor activation of phospholipase D.British journal of pharmacology · 2012Article
- Muscular effects of orexin A on the mouse duodenum: mechanical and electrophysiological studies.The Journal of physiology · 2011Article
- Ligand-induced internalization of the orexin OX(1) and cannabinoid CB(1) receptors assessed via N-terminal SNAP and CLIP-tagging.British journal of pharmacology · 2011Article
- State-dependent control of lumbar motoneurons by the hypocretinergic system.Experimental neurology · 2010Article
- Arachidonic acid release mediated by OX1 orexin receptors.British journal of pharmacology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposeOrexin (OX) receptors induce Ca2+ elevations via both receptor-operated Ca2+ channels (ROCs) and the "conventional" phospholipase C (PLC)-Ca2+ release-store-operated Ca2+ channel (SOC) pathways. In this study we assessed the ability of these different Ca2+ influx pathways to amplify OX1 receptor signalling to PLC in response to stimulation with the physiological ligand orexin-A. EXPERIMENTAL APPROACH: PLC activity was assessed in CHO cells stably expressing human OX1 receptors. KEY
resultsInhibition of total Ca2+ influx by reduction of the extracellular [Ca2+] to 1 microM effectively inhibited the receptor-stimulated PLC activity at low orexin-A concentrations (by 93% at 1 nM), and this effect was gradually reduced by higher orexin-A concentrations. A similar but weaker inhibitory effect (84% at 1 nM) was obtained on depolarization to approximately 0 mV, which disrupts most of the driving force for Ca2+ entry. The inhibitor of the OX1 receptor-activated ROCs, tetraethylammonium chloride (TEA), was somewhat less effective than the reduction in extracellular [Ca2+] at inhibiting PLC activation, probably because it only partially blocks ROCs. The partial inhibitor of both ROCs and SOCs, Mg2+, and the SOC inhibitors, dextromethorphan, SKF-96365 (1-[beta-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenethyl]-1H-imidazole HCL) and 2-APB (2-aminoethoxydiphenyl borate), inhibited PLC activity at low concentrations of orexin-A, but were not as effective as TEA. CONCLUSIONS AND IMPLICATIONS: Both ROCs and SOCs markedly amplify the OX(1) receptor-induced PLC response, but ROCs are more central for this response. These data indicate the crucial role of ROCs in orexin receptor signalling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.