ArticleThe Plant cell2006
Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 80 citations in OpenAlex.
- Splicing regulation by RS2Z36 controls ovary patterning and fruit growth in tomato.Plant molecular biology · 2026Article
- Alternative Splicing Variation: Accessing and Exploiting in Crop Improvement Programs.International journal of molecular sciences · 2023Review
- Review
- Phosphorylation mediated regulation of RNA splicing in plants.Frontiers in plant science · 2023Review
- Article
- Relevance and Regulation of Alternative Splicing in Plant Heat Stress Response: Current Understanding and Future Directions.Frontiers in plant science · 2022Review
- Comparative Phosphoproteomic Analysis Reveals the Response of Starch Metabolism to High-Temperature Stress in Rice Endosperm.International journal of molecular sciences · 2021Article
- Current Challenges in Studying Alternative Splicing in Plants: The Case ofFrontiers in plant science · 2020Article
- Highly ABA-Induced 1 (HAI1)-Interacting protein HIN1 and drought acclimation-enhanced splicing efficiency at intron retention sites.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Quantitative phosphoproteomic analyses provide evidence for extensive phosphorylation of regulatory proteins in the rhizobia-legume symbiosis.Plant molecular biology · 2019Article
- RNA Binding Motif Protein 48 Is Required for U12 Splicing and Maize Endosperm Differentiation.The Plant cell · 2019Article
- Subcellular Compartmentation of Alternatively Spliced Transcripts DefinesPlant physiology · 2018Article
- Ultrastructural and Molecular Analyses Reveal Enhanced Nucleolar Activity inFrontiers in plant science · 2018Article
- Identification of factors required for mThe New phytologist · 2017Article
- The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms.Plant cell reports · 2017Article
- Plant snRNP Biogenesis: A Perspective from the Nucleolus and Cajal Bodies.Frontiers in plant science · 2017Review
- Identification of potential cargo proteins of transportin protein AtTRN1 in Arabidopsis thaliana.Plant cell reports · 2016Article
- Dynamic Distribution and Interaction of the Arabidopsis SRSF1 Subfamily Splicing Factors.Plant physiology · 2016Article
- Proteome distribution between nucleoplasm and nucleolus and its relation to ribosome biogenesis in Arabidopsis thaliana.RNA biology · 2016Article
- Functional analysis of the three HMA4 copies of the metal hyperaccumulator Arabidopsis halleri.Journal of experimental botany · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serine/arginine-rich (SR) proteins are splicing regulators that share a modular structure consisting of one or two N-terminal RNA recognition motif domains and a C-terminal RS-rich domain. We investigated the dynamic localization of the Arabidopsis thaliana SR protein RSZp22, which, as we showed previously, distributes in predominant speckle-like structures and in the nucleolus. To determine the role of RSZp22 diverse domains in its nucleolar distribution, we investigated the subnuclear localization of domain-deleted mutant proteins. Our results suggest that the nucleolar localization of RSZp22 does not depend on a single targeting signal but likely involves different domains/motifs. Photobleaching experiments demonstrated the unrestricted dynamics of RSZp22 between nuclear compartments. Selective inhibitor experiments of ongoing cellular phosphorylation influenced the rates of exchange of RSZp22 between the different nuclear territories, indicating that SR protein mobility is dependent on the phosphorylation state of the cell. Furthermore, based on a leptomycin B- and fluorescence loss in photobleaching-based sensitive assay, we suggest that RSZp22 is a nucleocytoplasmic shuttling protein. Finally, with electron microscopy, we confirmed that RSp31, a plant-specific SR protein, is dynamically distributed in nucleolar cap-like structures upon phosphorylation inhibition. Our findings emphasize the high mobility of Arabidopsis SR splicing factors and provide insights into the dynamic relationships between the different nuclear compartments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.