ArticlePlant physiology2007
Tobacco nectaries express a novel NADPH oxidase implicated in the defense of floral reproductive tissues against microorganisms.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 97 citations in OpenAlex.
- Nectary starch degradation affects nectar chemical composition, but not nectar sugars, in Arabidopsis thaliana.Plant physiology · 2025Article
- In the nectar, there are answers: exploring the intersection of colored nectars and reactive oxygen species in manipulating pollinator behavior.The New phytologist · 2025Review
- Nectar peroxide: assessing variation among plant species, microbial tolerance, and effects on microbial community assembly.The New phytologist · 2025Article
- Protein elicitor PeVn1 induces resistance toFrontiers in microbiology · 2025Article
- Sugar Concentration, Nitrogen Availability, and Phylogenetic Factors Determine the Ability of Acinetobacter spp. and Rosenbergiella spp. to Grow in Floral Nectar.Microbial ecology · 2023Article
- Nectar compounds impact bacterial and fungal growth and shift community dynamics in a nectar analog.Environmental microbiology reports · 2023Article
- Evolutionary Analysis of Respiratory Burst Oxidase Homolog (RBOH) Genes in Plants and Characterization ofInternational journal of molecular sciences · 2023Article
- Nectar biosynthesis is conserved among floral and extrafloral nectaries.Plant physiology · 2021Article
- Article
- An integrated transcriptomics and metabolomics analysis of thePlant direct · 2019Article
- Effects on Plant Growth and Reproduction of a Peach R2R3-MYB Transcription Factor Overexpressed in Tobacco.Frontiers in plant science · 2019Article
- Generation of Superoxide by OeRbohH, a NADPH Oxidase Activity During Olive (Frontiers in plant science · 2019Article
- The major nectar protein of Brassica rapa is a non-specific lipid transfer protein, BrLTP2.1, with strong antifungal activity.Journal of experimental botany · 2018Article
- Congruence between PM HProtoplasma · 2018Article
- Sex-Dependent Variation of Pumpkin (Frontiers in plant science · 2018Article
- Nectar Analysis Throughout the GenusFrontiers in plant science · 2018Article
- Article
- An NADPH-Oxidase/Polyamine Oxidase Feedback Loop Controls Oxidative Burst Under Salinity.Plant physiology · 2016Article
- Floral Nectary Morphology and Proteomic Analysis of Nectar of Liriodendron tulipifera Linn.Frontiers in plant science · 2016Article
- The F-box protein COI1 functions upstream of MYB305 to regulate primary carbohydrate metabolism in tobacco (Nicotiana tabacum L. cv. TN90).Journal of experimental botany · 2014Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen peroxide produced from the nectar redox cycle was shown to be a major factor contributing to inhibition of most microbial growth in floral nectar; however, this obstacle can be overcome by the floral pathogen Erwinia amylovora. To identify the source of superoxide that leads to hydrogen peroxide accumulation in nectary tissues, nectaries were stained with nitroblue tetrazolium. Superoxide production was localized near nectary pores and inhibited by diphenylene iodonium but not by cyanide or azide, suggesting that NAD(P)H oxidase is the source of superoxide. Native PAGE assays demonstrated that NADPH (not NADH) was capable of driving the production of superoxide, diphenyleneiodonium chloride was an efficient inhibitor of this activity, but cyanide and azide did not inhibit. These results confirm that the production of superoxide was due to an NADPH oxidase. The nectary enzyme complex was distinct by migration on gels from the leaf enzyme complex. Temporal expression patterns demonstrated that the superoxide production (NADPH oxidase activity) was coordinated with nectar secretion, the expression of Nectarin I (a superoxide dismutase in nectar), and the expression of NOX1, a putative gene for a nectary NADPH oxidase that was cloned from nectaries and identified as an rbohD-like NADPH oxidase. Further, in situ hybridization studies indicated that the NADPH oxidase was expressed in the early stages of flower development although superoxide was generated at later stages (after Stage 10), implicating posttranslational regulation of the NADPH oxidase in the nectary.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.