ArticleJournal of bacteriology2007
Functional reconstitution of SdcS, a Na+-coupled dicarboxylate carrier protein from Staphylococcus aureus.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Pre-epidemic evolution of the MRSA USA300 clade and a molecular key for classification.Frontiers in cellular and infection microbiology · 2023Article
- Solvent accessibility changes in a NaThe Journal of biological chemistry · 2020Article
- Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications.FEMS microbiology letters · 2020Review
- Structure and Mechanism of the Divalent Anion/Na⁺ Symporter.International journal of molecular sciences · 2019Review
- Cultivation and characterization of Candidatus Nitrosocosmicus exaquare, an ammonia-oxidizing archaeon from a municipal wastewater treatment system.The ISME journal · 2017Article
- Structure and function of the divalent anion/NaNature communications · 2017Article
- Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques.European biophysics journal : EBJ · 2017Review
- Functional characterization of a Na+-dependent dicarboxylate transporter from Vibrio cholerae.The Journal of general physiology · 2014Article
- Functional characterization of SdcF from Bacillus licheniformis, a homolog of the SLC13 Na⁺/dicarboxylate transporters.The Journal of membrane biology · 2013Article
- Article
- Biochemical characterization of the C4-dicarboxylate transporter DctA from Bacillus subtilis.Journal of bacteriology · 2010Article
- Substrate-dependent proton antiport in neurotransmitter:sodium symporters.Nature chemical biology · 2010Article
- Functional characterization of a Na(+)-coupled dicarboxylate transporter from Bacillus licheniformis.Biochimica et biophysica acta · 2009Article
- Characterization of the dicarboxylate transporter DctA in Corynebacterium glutamicum.Journal of bacteriology · 2009Article
- Identification of conformationally sensitive amino acids in the Na(+)/dicarboxylate symporter (SdcS).Biochemistry · 2009Article
- Identification and characterization of the dicarboxylate uptake system DccT in Corynebacterium glutamicum.Journal of bacteriology · 2008Article
- Identification of a gene encoding a transporter essential for utilization of C4 dicarboxylates in Corynebacterium glutamicum.Applied and environmental microbiology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expressed SdcS in Escherichia coli and found it to have transport properties similar to those of its eukaryotic counterparts (J. A. Hall and A. M. Pajor, J. Bacteriol. 187:5189-5194, 2005). Here, we report the partial purification and subsequent reconstitution of functional SdcS into liposomes. These proteoliposomes exhibited succinate counterflow activity, as well as Na+ electrochemical-gradient-driven transport. Examination of substrate specificity indicated that the minimal requirement necessary for transport was a four-carbon terminal dicarboxylate backbone and that productive substrate-transporter interaction was sensitive to substitutions at the substrate C-2 and C-3 positions. Further analysis established that SdcS facilitates an electroneutral symport reaction having a 2:1 cation/dicarboxylate ratio. This study represents the first characterization of a reconstituted Na+-coupled DASS family member, thus providing an effective method to evaluate functional, as well as structural, aspects of DASS transporters in a system free of the complexities and constraints associated with native membrane environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.