ArticleBiophysical journal2007
Altered membrane dynamics of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow derived progenitor cells.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 65 citations in OpenAlex.
- Study on intracellular delivery of liposome encapsulated quantum dots using advanced fluorescence microscopy.Scientific reports · 2019Article
- Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses.Nanoscale · 2018Article
- Segmentation of 3D Trajectories Acquired by TSUNAMI Microscope: An Application to EGFR Trafficking.Biophysical journal · 2016Article
- Lateral Mobility and Nanoscale Spatial Arrangement of Chemokine-activated α4β1 Integrins on T Cells.The Journal of biological chemistry · 2016Article
- The effect of ligand affinity on integrins' lateral diffusion in cultured cells.European biophysics journal : EBJ · 2013Article
- Quantum dots: synthesis, bioapplications, and toxicity.Nanoscale research letters · 2012Article
- CD151 restricts the α6 integrin diffusion mode.Journal of cell science · 2012Article
- In vivo imaging of embryonic stem cell therapy.European journal of nuclear medicine and molecular imaging · 2011Review
- Entrapment in phospholipid vesicles quenches photoactivity of quantum dots.International journal of nanomedicine · 2011Article
- Real-time optical imaging using quantum dot and related nanocrystals.Nanomedicine (London, England) · 2010Review
- Probing cellular events, one quantum dot at a time.Nature methods · 2010Review
- Stem cell tracking by nanotechnologies.International journal of molecular sciences · 2010Review
- Physicochemical control of adult stem cell differentiation: shedding light on potential molecular mechanisms.Journal of biomedicine & biotechnology · 2010Review
- Dynamic partitioning of a glycosyl-phosphatidylinositol-anchored protein in glycosphingolipid-rich microdomains imaged by single-quantum dot tracking.Traffic (Copenhagen, Denmark) · 2009Article
- Chondrogenic differentiation of human mesenchymal stem cells on oriented nanofibrous scaffolds: engineering the superficial zone of articular cartilage.Tissue engineering. Part A · 2009Article
- Regulation of cell cytoskeleton and membrane mechanics by electric field: role of linker proteins.Biophysical journal · 2009Article
- Bioconjugated quantum dots for in vivo molecular and cellular imaging.Advanced drug delivery reviews · 2008Review
- Integrin-adhesion ligand bond formation of preosteoblasts and stem cells in three-dimensional RGD presenting matrices.Biomacromolecules · 2008Article
- Modulation of cellular mechanics during osteogenic differentiation of human mesenchymal stem cells.Biophysical journal · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Functionalized quantum dots offer several advantages for tracking the motion of individual molecules on the cell surface, including selective binding, precise optical identification of cell surface molecules, and detailed examination of the molecular motion without photobleaching. We have used quantum dots conjugated with integrin antibodies and performed studies to quantitatively demonstrate changes in the integrin dynamics during osteogenic differentiation of human bone marrow derived progenitor cells (BMPCs). Consistent with the unusually strong BMPC adhesion previously observed, integrins on the surface of undifferentiated BMPC were found in clusters and the lateral diffusion was slow (e.g., approximately 10(-11) cm2/s). At times as early as those after a 3-day incubation in the osteogenic differentiation media, the integrin diffusion coefficients increased by an order of magnitude, and the integrin dynamics became indistinguishable from that measured on the surface of terminally differentiated human osteoblasts. Furthermore, microfilaments in BMPCs consisted of atypically thick bundles of stress fibers that were responsible for restricting the integrin lateral mobility. Studies using laser optical tweezers showed that, unlike fully differentiated osteoblasts, the BMPC cytoskeleton is weakly associated with its cell membrane. Based on these findings, it appears likely that the altered integrin dynamics is correlated with BMPC differentiation and that the integrin lateral mobility is restricted by direct links to microfilaments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.