ArticleBiophysical journal2007
FRET study of membrane proteins: determination of the tilt and orientation of the N-terminal domain of M13 major coat protein.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Fluorescent Probes and Quenchers in Studies of Protein Folding and Protein-Lipid Interactions.Chemical record (New York, N.Y.) · 2024Review
- Lipid-Protein Interactions in Plasma Membrane Organization and Function.Annual review of biophysics · 2022Review
- The Secret Lives of Fluorescent Membrane Probes as Revealed by Molecular Dynamics Simulations.Molecules (Basel, Switzerland) · 2020Review
- Visualization of Protein Interactions in Living Cells.Self/nonself · 2011Article
- Viruses: incredible nanomachines. New advances with filamentous phages.European biophysics journal : EBJ · 2010Review
- Profiling of dynamics in protein-lipid-water systems: a time-resolved fluorescence study of a model membrane protein with the label BADAN at specific membrane depths.European biophysics journal : EBJ · 2010Article
- Orientation and dynamics of transmembrane peptides: the power of simple models.European biophysics journal : EBJ · 2010Review
- Molecular dynamics simulations reveal that AEDANS is an inert fluorescent probe for the study of membrane proteins.European biophysics journal : EBJ · 2010Article
- Influence of whole-body dynamics on 15N PISEMA NMR spectra of membrane proteins: a theoretical analysis.Biophysical journal · 2009Article
- Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion.Nature structural & molecular biology · 2008Article
- Site-directed fluorescence labeling of a membrane protein with BADAN: probing protein topology and local environment.Biophysical journal · 2008Article
- A novel switch region regulates H-ras membrane orientation and signal output.The EMBO journal · 2008Article
- Visualization of protein interactions in living cells.Advances in experimental medicine and biology · 2008Review
- Structure of membrane-embedded M13 major coat protein is insensitive to hydrophobic stress.Biophysical journal · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A formalism for membrane protein structure determination was developed. This method is based on steady-state FRET data and information about the position of the fluorescence maxima on site-directed fluorescent labeled proteins in combination with global data analysis utilizing simulation-based fitting. The methodology was applied to determine the structural properties of the N-terminal domain of the major coat protein from bacteriophage M13 reconstituted into unilamellar DOPC/DOPG (4:1 mol/mol) vesicles. For our purpose, the cysteine mutants A7C, A9C, N12C, S13C, Q15C, A16C, S17C, and A18C in the N-terminal domain of this protein were produced and specifically labeled with the fluorescence probe AEDANS. The energy transfer data from the natural Trp-26 to AEDANS were analyzed assuming a two-helix protein model. Furthermore, the polarity Stokes shift of the AEDANS fluorescence maxima is taken into account. As a result the orientation and tilt of the N-terminal protein domain with respect to the bilayer interface were obtained, showing for the first time, to our knowledge, an overall alpha-helical protein conformation from amino acid residues 12-46, close to the protein conformation in the intact phage.
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