ArticleGene2007
Multiple roles for MSH2 in the repair of a deletion mutation directed by modified single-stranded oligonucleotides.
Article in Gene, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Genetic spell-checking: gene editing using single-stranded DNA oligonucleotides.Plant biotechnology journal · 2016Review
- Emerging gene editing strategies for Duchenne muscular dystrophy targeting stem cells.Frontiers in physiology · 2014Review
- Article
- Progress and prospects: oligonucleotide-directed gene modification in mouse embryonic stem cells: a route to therapeutic application.Gene therapy · 2011Review
- An update on targeted gene repair in mammalian cells: methods and mechanisms.Journal of biomedical science · 2011Review
- Parameters of oligonucleotide-mediated gene modification in mouse ES cells.Journal of cellular and molecular medicine · 2010Article
- Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides.Nucleic acids research · 2009Article
- DNA breakage associated with targeted gene alteration directed by DNA oligonucleotides.Mutation research · 2009Article
- Genetic correction of splice site mutation in purified and enriched myoblasts isolated from mdx5cv mice.BMC molecular biology · 2009Article
- Oligonucleotide-mediated gene targeting in human hepatocytes: implications of mismatch repair.Oligonucleotides · 2008Article
- Simultaneous targeted exchange of two nucleotides by single-stranded oligonucleotides clusters within a region of about fourteen nucleotides.BMC molecular biology · 2008Article
- Progress and prospects: targeted gene alteration (TGA).Gene therapy · 2007Review
- Oligonucleotide transformation of yeast reveals mismatch repair complexes to be differentially active on DNA replication strands.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The mechanism by which modified single-stranded oligonucleotides (MSSOs) direct base changes in genes is not completely understood, but there is evidence that DNA damage, repair and cell cycle checkpoint proteins are involved in the targeted nucleotide exchange (TNE) process. We are interested in the role of the mismatch repair protein, Msh2 in the correction of a frameshift mutation in both yeast and mammalian cells. We show that this protein exerts different and opposing influences on the TNE reaction in MSH2 deficient yeast compared to MSH2(-/-) mammalian cells and in wild-type cells that have RNAi silenced Msh2. Data from yeast show a 10-fold decrease in the targeting frequency whereas mammalian cells have an elevated correction frequency. These results show that in yeast this protein is required for efficient targeting and may play a role in mismatch recognition and repair. In mammalian cells, Msh2 plays a suppressive role in TNE reaction by either precluding the oligonucleotide annealing to the target gene or by maintenance of a cell cycle checkpoint induced by the MSSO itself. These results reveal that the mechanism of TNE between yeast and mammalian cells is not conserved, and demonstrate that the suppression of the TNE reaction can be bypassed using RNAi against MSH2 designed to knockdown its expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.