ArticleProceedings of the National Academy of Sciences of the United States of America1991
Newly synthesized RNA: simultaneous measurement in intact cells of transcription rates and RNA stability of insulin-like growth factor I, actin, and albumin in growth hormone-stimulated hepatocytes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
- Trial
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- Determining mRNA Stability by Metabolic RNA Labeling and Chemical Nucleoside Conversion.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Metabolic labeling and recovery of nascent RNA to accurately quantify mRNA stability.Methods (San Diego, Calif.) · 2017Article
- Critical appraisal of the role of serum albumin in cardiovascular disease.Biomarker research · 2017Review
- Temporally and spatially restricted gene expression profiling.Current genomics · 2014Article
- Genome-wide survey of interindividual differences of RNA stability in human lymphoblastoid cell lines.Scientific reports · 2013Article
- Endo-siRNAs depend on a new isoform of loquacious and target artificially introduced, high-copy sequences.The EMBO journal · 2009Article
- Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels.Molecular and cellular biology · 1999Article
- Overexpression of growth hormone affects alternatively spliced IGF-I mRNA expression in oMt1a-oGH transgenic mice.Transgenic research · 1998Article
- Messenger RNA deadenylylation precedes decapping in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- mRNA stability in mammalian cells.Microbiological reviews · 1995Review
- Vitamin B6 modulates expression of albumin gene by inactivating tissue-specific DNA-binding protein in rat liver.The Biochemical journal · 1995Article
- Suppression of albumin enhancer activity by H-ras and AP-1 in hepatocyte cell lines.Molecular and cellular biology · 1994Article
- Differential regulation of multiple gap junction transcripts and proteins during rat liver regeneration.The Journal of cell biology · 1993Article
- Mammal cells double their total RNAs against diabetes, ischemia reperfusion and malaria-induced oxidative stress.Molecular medicine (Cambridge, Mass.)Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The levels of several RNA transcripts in cultured hepatocytes are regulated by transcriptional and post-transcriptional mechanisms and are affected by growth hormone and insulin. We assessed the effects of these hormones on transcription rates and the stability of insulin-like growth factor I, actin, and albumin transcripts in intact cells of primary cultures of rat hepatocytes by analyzing thiol-labeled, newly synthesized RNA isolated by mercurated agarose affinity chromatography. The application of this concept to the measurement of transcript stability is presented in detail. The data indicate that growth hormone stimulates the transcription rates of insulin-like growth factor I, actin, and albumin genes. The stability of all three transcripts, particularly albumin, appears to be lower in growth hormone-containing medium than it is in insulin-containing medium. The experiments indicate that the rates of transcription and/or degradation of albumin mRNA are influenced by hormonal treatment. However, the cells maintain roughly constant albumin transcript levels independent of hormone treatment by compensatory changes in the rates of transcription and degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.