ArticleGenetics2007
Chi hotspot activity in Escherichia coli without RecBCD exonuclease activity: implications for the mechanism of recombination.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 20 citations in OpenAlex.
- RecBCD enzyme: mechanistic insights from mutants of a complex helicase-nuclease.Microbiology and molecular biology reviews : MMBR · 2023Review
- RecBCD enzyme and Chi recombination hotspots as determinants of self vs. non-self: Myths and mechanisms.Advances in genetics · 2022Article
- Physiology of the read-write genome.The Journal of physiology · 2014Review
- Characterization of an unusual bipolar helicase encoded by bacteriophage T5.Nucleic acids research · 2013Article
- How RecBCD enzyme and Chi promote DNA break repair and recombination: a molecular biologist's view.Microbiology and molecular biology reviews : MMBR · 2012Review
- Identification of hot and cold spots in genome of Mycobacterium tuberculosis using Shewhart Control Charts.Scientific reports · 2012Article
- RecBCD enzyme and the repair of double-stranded DNA breaks.Microbiology and molecular biology reviews : MMBR · 2008Review
- Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.Genes & development · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The major pathway of genetic recombination and DNA break repair in Escherichia coli requires RecBCD enzyme, a complex nuclease and DNA helicase regulated by Chi sites (5'-GCTGGTGG-3'). During its unwinding of DNA containing Chi, purified RecBCD enzyme has two alternative nucleolytic reactions, depending on the reaction conditions: simple nicking of the Chi-containing strand at Chi or switching of nucleolytic degradation from the Chi-containing strand to its complement at Chi. We describe a set of recC mutants with a novel intracellular phenotype: retention of Chi hotspot activity in genetic crosses but loss of detectable nucleolytic degradation as judged by the growth of mutant T4 and lambda phages and by assay of cell-free extracts. We conclude that RecBCD enzyme's nucleolytic degradation of DNA is not necessary for intracellular Chi hotspot activity and that nicking of DNA by RecBCD enzyme at Chi is sufficient. We discuss the bearing of these results on current models of RecBCD pathway recombination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.