ArticleThe EMBO journal1991
An indicator gene for detection of germline retrotransposition in transgenic Drosophila demonstrates RNA-mediated transposition of the LINE I element.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Review
- Translesion DNA polymerases Rev1 and PolH promote LTR-retrotransposon transcription by safeguarding Pol II occupancy in both germline and somatic tissues of Drosophila.Nucleic acids research · 2025Article
- Ongoing endeavors to detect mobilization of transposable elements.BMB reports · 2022Review
- Tracing Mobile DNAs: From Molecular to Population Scales.Frontiers in plant science · 2022Review
- Article
- Article
- Retrotransposition of marked SVA elements by human L1s in cultured cells.Human molecular genetics · 2011Article
- The L1 retrotransposition assay: a retrospective and toolkit.Methods (San Diego, Calif.) · 2009Review
- Distinct mechanisms for trans-mediated mobilization of cellular RNAs by the LINE-1 reverse transcriptase.Genome research · 2007Article
- Natural epigenetic protection against the I-factor, a Drosophila LINE retrotransposon, by remnants of ancestral invasions.PloS one · 2007Article
- Article
- A Tetrahymena thermophila ribozyme-based indicator gene to detect transposition of marked retroelements in mammalian cells.Nucleic acids research · 2002Article
- Transplantation of target site specificity by swapping the endonuclease domains of two LINEs.The EMBO journal · 2002Article
- Artificial and epigenetic regulation of the I factor, a nonviral retrotransposon of Drosophila melanogaster.Genetics · 2000Article
- Retrotransposition of the I factor, a non-long terminal repeat retrotransposon of Drosophila, generates tandem repeats at the 3' end.Nucleic acids research · 2000Article
- Cosuppression of I transposon activity in Drosophila by I-containing sense and antisense transgenes.Genetics · 1999Article
- Article
- Copy number control of a transposable element, the I factor, a LINE-like element in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Evidence for a multistep control in transposition of I factor in Drosophila melanogaster.Genetics · 1998Article
- A genetically marked I element in Drosophila melanogaster can be mobilized when ORF2 is provided in trans.Genetics · 1998Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have marked a cloned Drosophila transposable element--the I element--with an engineered neomycin-containing indicator gene, whose expression is conditioned by passage of the transposon through an RNA intermediate. Mobility of the marked element introduced into Drosophila as a transgene could be detected in vivo, upon in toto selection of developing embryos on G418-containing medium. For resistant individuals, Southern blot analysis and nucleotide sequencing after PCR amplification disclosed transposition of the marked element into new loci, with target site duplications and splicing out of the intron in the indicator gene. It demonstrates that the I element, which is closely related to the widespread mammalian LINEs, transposes through an RNA intermediate, as up to now only conjectured from sequence singularities of this class of 'non-viral retrotransposons'. The developed indicator gene provides a potent new genetic tool for detection and quantitative analysis of retrotransposon mobility and its regulation as it occurs in vivo.
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