ArticleThe EMBO journal1991
Specific expression of the tobacco Tnt1 retrotransposon in protoplasts.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 186 citations in OpenAlex.
- Metabolomic adaptations and genetic polymorphism in ecopopulations ofFrontiers in plant science · 2025Article
- Genomic imbalance modulates transposable element expression in maize.Plant communications · 2023Article
- Triggers and mediators of epigenetic remodeling in plants.Frontiers in epigenetics and epigenomics · 2023Review
- Diverse and mobile: eccDNA-based identification of carrot low-copy-number LTR retrotransposons active in callus cultures.The Plant journal : for cell and molecular biology · 2022Article
- Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress.BMC plant biology · 2021Article
- Dynamic Patterns of Transcript Abundance of Transposable Element Families in Maize.G3 (Bethesda, Md.) · 2019Article
- Retrotransposons in Plant Genomes: Structure, Identification, and Classification through Bioinformatics and Machine Learning.International journal of molecular sciences · 2019Review
- Agrobacterium-mediated Tnt1 mutagenesis of moss protonemal filaments and generation of stable mutants with impaired gametophyte.Molecular genetics and genomics : MGG · 2019Article
- Transcriptional control and exploitation of an immune-responsive family of plant retrotransposons.The EMBO journal · 2018Article
- Genome-wide analysis of LTR-retrotransposons in oil palm.BMC genomics · 2015Article
- Senescence Meets Dedifferentiation.Plants (Basel, Switzerland) · 2015Review
- Control of transposable elements in Arabidopsis thaliana.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Review
- Tissue culture-induced heritable genomic variation in rice, and their phenotypic implications.PloS one · 2014Article
- Transcriptional activity, chromosomal distribution and expression effects of transposable elements in Coffea genomes.PloS one · 2013Article
- Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications.Plant cell reports · 2012Review
- Article
- The stem cell state in plant development and in response to stress.Frontiers in plant science · 2011Article
- Transcriptional activity of transposable elements in maize.BMC genomics · 2010Article
- Retrotransposon vectors for gene delivery in plants.Mobile DNA · 2010Article
- Potential impact of stress activated retrotransposons on genome evolution in a marine diatom.BMC genomics · 2009Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Tnt1 transposable element of tobacco belongs to the retrotransposon family and shares the structural features of viral retroelements including two long terminal repeats (LTRs) which are known to contain promoter regions. We show that two Tnt1 RNAs of 5.2 and 6.5 kb can be found. The 5.2 kb RNA matches with the size of the Tnt1 elements so far isolated (5.3 kb), whilst the evidence suggests that the 6.5 kb RNA could be a chimaeric RNA initiated in a gene in which Tnt1 has inserted. The Tnt1 5.2 kb RNA starts in the LTR, and the LTR can promote the expression of a translational LTR-beta-glucuronidase (GUS) fusion at a high level in transient expression assays. The Tnt1 5.2 kb RNA and the LTR-GUS fusion of transgenic tobacco plants are specifically expressed in leaf-derived protoplasts whereas they are not expressed in leaf tissue. The 5.2 kb RNA is also transcribed at low levels in roots. This RNA is induced after 2 h of maceration in the protoplast isolation medium, and its level declines rapidly after protoplast isolation. The induction requires only the presence of cell wall hydrolases, and is independent of wounding and plasmolysis. The induction of Tnt1 expression is not mediated by typical oligosaccharide elicitors released from the cell wall known to mediate defense gene responses. Tnt1 transcription features provide a first example of tissue culture-induced mutagenesis in plants and a molecular basis for some of the somaclonal variation events.
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