ArticleBiophysical journal1991
Quantitative video microscopy of patch clamped membranes stress, strain, capacitance, and stretch channel activation.
Article in Biophysical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
95 citing papers in PubMed, 259 citations in OpenAlex.
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- Membrane-coated glass electrodes for stable, low-noise electrophysiology recordings in Drosophila central neurons.Journal of neuroscience methods · 2024Article
- Observing mechanosensitive channels in action in living bacteria.Biophysical reports · 2024Article
- How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels.International journal of molecular sciences · 2022Review
- TRPV Protein Family-From Mechanosensing to Cancer Invasion.Biomolecules · 2021Review
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- Voltage Does Not Drive Prestin (SLC26a5) Electro-Mechanical Activity at High Frequencies Where Cochlear Amplification Is Best.iScience · 2019Article
- "Force-From-Lipids" mechanosensation in Corynebacterium glutamicum.Biophysical reviews · 2019Review
- Activation of Piezo1 but Not NaUltrasound in medicine & biology · 2018Article
- Control of cell behaviour through nanovibrational stimulation: nanokicking.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2018Review
- Studying Mechanosensitivity of Two-Pore Domain KMethods in molecular biology (Clifton, N.J.) · 2018Article
- A Dynamic Biochemomechanical Model of Geometry-Confined Cell Spreading.Biophysical journal · 2017Article
- Whole-GUV patch-clamping.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Application of Environmental Scanning Electron Microscope-Nanomanipulation System on Spheroplast Yeast Cells Surface Observation.Scanning · 2017Article
- Energy Output from a Single Outer Hair Cell.Biophysical journal · 2016Article
- Adenosine monophosphate-activated protein kinase activation and suppression of inflammatory response by cell stretching in rabbit synovial fibroblasts.Molecular and cellular biochemistry · 2016Article
- Influence of Global and Local Membrane Curvature on Mechanosensitive Ion Channels: A Finite Element Approach.Membranes · 2016Article
- Cardiac Mechano-Gated Ion Channels and Arrhythmias.Circulation research · 2016Review
- Mechanical sensitivity of Piezo1 ion channels can be tuned by cellular membrane tension.eLife · 2015Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Membrane patches from chick skeletal muscle were stretched by applying controlled suction or pressure to the pipette. From images of the patch, the patch dimensions (area and radius of curvature) were computed by nonlinear regression of the images to a geometric model. With no applied pressure, patch membranes are nearly planar and normal to the wall of the pipette. With increasing pressure gradients, the patch bulges, the radius of curvature decreases, and the area increases. The patch capacitance changes in exact proportion to the change in area at a rate of 0.7 microF/cm2. The increase in area is due to a flow of lipid (with perhaps small amounts of diffusible protein) along the walls of the pipette into the patch. The flow is reversible with a relaxation of the pressure gradient. The area elastic constant of the membrane is approximately 50 dyn/cm, insensitive to cytochalasin B and probably represents the elasticity of the underlying spectrin/dystrophin network. Simultaneous measurements of stretch activated (SA) ion channel activity in the patch showed that the sensitivity of channels from different patches, although different when calculated as a function of applied pressure, was the same when calculated as a function of tension. Because patch lipid is free to flow, and hence stress-free in the steady state, SA channels must be activated by tension in the cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.