ArticleInternational journal of legal medicine2007
Development of species identification tests targeting the 16S ribosomal RNA coding region in mitochondrial DNA.
Article in International journal of legal medicine, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 56 citations in OpenAlex.
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- Current issues for mammalian species identification in forensic science: a review.International journal of legal medicine · 2021Review
- Evaluation of Parameters for Estimating the Postmortem Interval of Skeletal Remains Using Bovine Femurs: A Pilot Study.Diagnostics (Basel, Switzerland) · 2020Article
- Identification of a broad spectrum of mammalian and avian species using the short fragment of the mitochondrially encoded cytochrome b gene.Forensic science, medicine, and pathology · 2019Article
- Establishment of widely applicable DNA extraction methods to identify the origins of crude drugs derived from animals using molecular techniques.Journal of natural medicines · 2019Article
- DNA survival and physical and histological properties of heat-induced alterations in burnt bones.International journal of legal medicine · 2014Article
- Pet fur or fake fur? A forensic approach.Investigative genetics · 2014Article
- Authentication of animal signatures in traditional Chinese medicine of Lingyang Qingfei Wan using routine molecular diagnostic assays.Molecular biology reports · 2014Article
- Identification of species by multiplex analysis of variable-length sequences.Nucleic acids research · 2010Article
- Developing equine mtDNA profiling for forensic application.International journal of legal medicine · 2010Article
- DNA typing in wildlife crime: recent developments in species identification.Forensic science, medicine, and pathology · 2010Review
- Forensic botany: species identification of botanical trace evidence using a multigene barcoding approach.International journal of legal medicine · 2009Article
- Forensic species identification based on size variation of mitochondrial DNA hypervariable regions.International journal of legal medicine · 2009Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This report describes the development of a species testing system based on the diversity of nucleotide sequences in mitochondrial DNA (mtDNA) among species. Five species, human, cow, pig, dog, and cat, were considered. The partial nucleotide sequences in 16S ribosomal RNA coding region were chosen as the target for discriminating the species. The sequence diversities of this approximately 400 bp long region ranged from 15.7 to 24.1% among the five species. Sequencing analysis of this target on 50 individuals of each species (53 for dogs) revealed that the nucleotide sequences were well preserved within species. Species-specific PCR for each species was also designed, and satisfactory results with regard to both sensitivity and specificity were obtained. A validation study with DNA extracted from bovine bone exposed to the environment revealed that the PCRs designed in this study worked correctly. From the results obtained, it was shown that this testing system could be a good tool for species identification. One successful case report is also demonstrated.
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