ArticleNucleic acids research2006
Stimulation of MCM helicase activity by a Cdc6 protein in the archaeon Thermoplasma acidophilum.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Unwinding 20 Years of the Archaeal Minichromosome Maintenance Helicase.Journal of bacteriology · 2020Review
- Article
- Characterization of the MCM homohexamer from the thermoacidophilic euryarchaeon Picrophilus torridus.Scientific reports · 2015Article
- Transfer RNA methyltransferases from Thermoplasma acidophilum, a thermoacidophilic archaeon.International journal of molecular sciences · 2014Article
- Activation of the MCM helicase from the thermophilic archaeon, Thermoplasma acidophilum by interactions with GINS and Cdc6-2.Extremophiles : life under extreme conditions · 2014Article
- Characterization of the replication initiator Orc1/Cdc6 from the Archaeon Picrophilus torridus.Journal of bacteriology · 2014Article
- The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication.Extremophiles : life under extreme conditions · 2011Article
- Article
- Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus.Extremophiles : life under extreme conditions · 2010Article
- The Mcm complex: unwinding the mechanism of a replicative helicase.Microbiology and molecular biology reviews : MMBR · 2009Review
- Biochemical characterization of the minichromosome maintenance protein from the archaeon Thermoplasma acidophilum.Extremophiles : life under extreme conditions · 2009Article
- The origin recognition complex protein family.Genome biology · 2009Review
- Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity.Nucleic acids research · 2008Article
- Cryo-electron microscopy reveals a novel DNA-binding site on the MCM helicase.The EMBO journal · 2008Article
- The Methanothermobacter thermautotrophicus Cdc6-2 protein, the putative helicase loader, dissociates the minichromosome maintenance helicase.Journal of bacteriology · 2008Article
- Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum.The open biochemistry journal · 2008Article
- DNA unwinding assay using streptavidin-bound oligonucleotides.BMC molecular biology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replicative DNA helicases are ring-shaped hexamers that play an essential role in chromosomal DNA replication. They unwind the two strands of the duplex DNA and provide the single-stranded (ss) DNA substrate for the polymerase. The minichromosome maintenance (MCM) proteins are thought to function as the replicative helicases in eukarya and archaea. The proteins of only a few archaeal organisms have been studied and revealed that although all have similar amino acid sequences and overall structures they differ in their biochemical properties. In this report the biochemical properties of the MCM protein from the archaeon Thermoplasma acidophilum is described. The enzyme has weak helicase activity on a substrate containing only a 3'-ssDNA overhang region and the protein requires a forked DNA structure for efficient helicase activity. It was also found that the helicase activity is stimulated by one of the two T.acidophilum Cdc6 homologues. This is an interesting observation as it is in sharp contrast to observations made with MCM and Cdc6 homologues from other archaea in which the helicase activity is inhibited when bound to Cdc6.
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