ArticleBiology of reproduction2007
Production of F1 interspecies hybrid offspring with cryopreserved sperm from a live-bearing fish, the swordtail Xiphophorus helleri.
Article in Biology of reproduction, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- An Open-Hardware Insemination Device for Small-Bodied Live-Bearing Fishes to Support Development and Use of Germplasm Repositories.Animals : an open access journal from MDPI · 2022Article
- On-Site Capabilities of a Mobile Laboratory for Aquatic Germplasm Cryopreservation.North American journal of aquaculture · 2019Article
- Development of germplasm repositories to assist conservation of endangered fishes: Examples from small-bodied livebearing fishes.Theriogenology · 2019Article
- Workshop report: Cryopreservation of aquatic biomedical models.Cryobiology · 2019Article
- The role of alkalinization-induced Ca2+ influx in sperm motility activation of a viviparous fish Redtail Splitfin (Xenotoca eiseni).Biology of reproduction · 2018Article
- Design and Cost Analysis of a Self-contained Mobile Laboratory for Commercial-scale Aquatic Species Cryopreservation.Journal of the World Aquaculture Society · 2018Article
- Production of live young with cryopreserved sperm from the endangered livebearing fish Redtail Splitfin (Xenotoca eiseni, Rutter, 1896).Animal reproduction science · 2018Article
- Cryopreservation of sperm bundles (spermatozeugmata) from endangered livebearing goodeids.Cryobiology · 2018Article
- Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, Xenotoca eiseni.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2018Article
- Cryobanking of aquatic species.Aquaculture (Amsterdam, Netherlands) · 2017Article
- Article
- Offspring production with cryopreserved sperm from a live-bearing fish Xiphophorus maculatus and implications for female fecundity.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2012Article
- Production of F₁ offspring with vitrified sperm from a live-bearing fish, the green swordtail Xiphophorus hellerii.Zebrafish · 2011Article
- Outlook for development of high-throughput cryopreservation for small-bodied biomedical model fishes.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2011Article
- Evaluation of cryoprotectant and cooling rate for sperm cryopreservation in the euryhaline fish medaka Oryzias latipes.Cryobiology · 2010Article
- Sperm motility initiation and duration in a euryhaline fish, medaka (Oryzias latipes).Theriogenology · 2009Article
- Sperm cryopreservation of a live-bearing fish, Xiphophorus couchianus: male-to-male variation in post-thaw motility and production of F(1) hybrid offspring.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2009Article
- Current status of sperm cryopreservation in biomedical research fish models: zebrafish, medaka, and Xiphophorus.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Despite study of sperm cryopreservation in more than 200 fish species, production of broods from cryopreserved sperm in live-bearing fish has not been demonstrated. This has not been due to a lack of effort, but instead is a result of the unique morphology, biology, and biochemistry of reproduction in viviparous fishes. For example, sperm of Xiphophorus helleri have a cylindrical nucleus, can swim for days after being activated, have glycolytic capabilities, and can reside in the female reproduction tract for months before fertilization. These traits are not found in fishes with external fertilization. The long-standing research use of the genus Xiphophorus has led to development of over 60 pedigreed lines among the 26 species maintained around the world. These species and lines serve as contemporary models in medical research, although they must be maintained as live populations. Previous attempts at establishing sperm cryopreservation protocols for Xiphophorus have not produced live young. To address this we have been studying the parameters surrounding cryobiology of Xiphophorus sperm and applying this information to an improved understanding of internal fertilization and reproduction. Here we report the first successful fertilization and offspring production by cryopreserved sperm in any live-bearing fish. This claim is supported by our use of artificial insemination between two species that yield distinct hybrid offspring to verify paternity via cryopreserved sperm. We provide a practical approach for preservation of valuable genetic resources from live-bearing fish species, a group that is rapidly being lost due to destruction of native habitats.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.