ArticleMolecular biology of the cell2007
Micromanipulation studies of chromatin fibers in Xenopus egg extracts reveal ATP-dependent chromatin assembly dynamics.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 78 citations in OpenAlex.
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- Single-Molecule Approaches to Study DNA Condensation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Monitoring the compaction of single DNA molecules inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nucleus size and its effect on nucleosome stability in living cells.Biophysical journal · 2022Article
- DNA-binding proteins studied by mechanical manipulation and AFM imaging of single DNA molecules.Biophysics reports · 2022Article
- Making Mitotic Chromosomes in a Test Tube.Epigenomes · 2022Review
- Shaping centromeres to resist mitotic spindle forces.Journal of cell science · 2022Article
- A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins.Molecules (Basel, Switzerland) · 2021Article
- The Accidental Ally: Nucleosome Barriers Can Accelerate Cohesin-Mediated Loop Formation in Chromatin.Biophysical journal · 2020Article
- Article
- White spot syndrome viral protein VP9 alters the cellular higher-order chromatin structure.FASEB bioAdvances · 2020Article
- DNA-segment-capture model for loop extrusion by structural maintenance of chromosome (SMC) protein complexes.Nucleic acids research · 2019Article
- Transfer-matrix calculations of the effects of tension and torque constraints on DNA-protein interactions.Nucleic acids research · 2018Article
- Mechanical evolution of DNA double-strand breaks in the nucleosome.PLoS computational biology · 2018Article
- Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers.Biophysics reports · 2018Article
- Oligomerization and ATP stimulate condensin-mediated DNA compaction.Scientific reports · 2017Article
- Tension sensors reveal how the kinetochore shares its load.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied assembly of chromatin using Xenopus egg extracts and single DNA molecules held at constant tension by using magnetic tweezers. In the absence of ATP, interphase extracts were able to assemble chromatin against DNA tensions of up to 3.5 piconewtons (pN). We observed force-induced disassembly and opening-closing fluctuations, indicating our experiments were in mechanochemical equilibrium. Roughly 50-nm (150-base pair) lengthening events dominated force-driven disassembly, suggesting that the assembled fibers are chiefly composed of nucleosomes. The ATP-depleted reaction was able to do mechanical work of 27 kcal/mol per 50 nm step, which provides an estimate of the free energy difference between core histone octamers on and off DNA. Addition of ATP led to highly dynamic behavior with time courses exhibiting processive runs of assembly and disassembly not observed in the ATP-depleted case. With ATP present, application of forces of 2 pN led to nearly complete fiber disassembly. Our study suggests that ATP hydrolysis plays a major role in nucleosome rearrangement and removal and that chromatin in vivo may be subject to highly dynamic assembly and disassembly processes that are modulated by DNA tension.
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