ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2006
High sensitivity to neuromodulator-activated signaling pathways at physiological [K+] of confocally imaged respiratory center neurons in on-line-calibrated newborn rat brainstem slices.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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75 citing papers in PubMed, 140 citations in OpenAlex.
- Roflumilast, a phosphodiesterase-4 (PDE4) inhibitor, induces respiratory frequency plasticity that is resistant to inflammation in neonatal rat in vitro preparations.Respiratory physiology & neurobiology · 2025Article
- Intrinsic responses to hypoxia and hypercapnia of neurons in the cardiorespiratory center of the ventral medulla of newborn rats.Pflugers Archiv : European journal of physiology · 2025Article
- A muscarinic, GIRK channel-mediated inhibition of inspiratory-related XII nerve motor output emerges in early postnatal development in mice.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Arginine vasopressin potentiates inspiratory bursting in hypoglossal motoneurons of neonatal mice.Respiratory physiology & neurobiology · 2023Article
- Maternal opioids age-dependently impair neonatal respiratory control networks.Frontiers in physiology · 2023Article
- Glycine and GABAA receptors suppressively regulate the inspiratory-related calcium rise in the thoracic inspiratory cells of the neonatal rat.The journal of physiological sciences : JPS · 2022Article
- A brainstem preparation allowing simultaneous access to respiratory motor output and cellular properties of motoneurons in American bullfrogs.The Journal of experimental biology · 2022Article
- Dynamic Rhythmogenic Network States Drive Differential Opioid Responses in theThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Chronic BDNF simultaneously inhibits and unmasks superficial dorsal horn neuronal activity.Scientific reports · 2021Article
- KCNQ Current Contributes to Inspiratory Burst Termination in the Pre-Bötzinger Complex of Neonatal RatsFrontiers in physiology · 2021Article
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- Time and dose-dependent impairment of neonatal respiratory motor activity after systemic inflammation.Respiratory physiology & neurobiology · 2020Article
- Optogenetic analysis of respiratory neuronal networks in the ventral medulla of neonatal rats producing channelrhodopsin in Phox2b-positive cells.Pflugers Archiv : European journal of physiology · 2019Article
- Muscarinic Modulation of Morphologically Identified Glycinergic Neurons in the Mouse PreBötzinger Complex.Frontiers in cellular neuroscience · 2019Article
- Development of a Novel FIJI-Based Method to Investigate Neuronal Circuitry in Neonatal Mice.Developmental neurobiology · 2018Article
- Release of ATP by pre-Bötzinger complex astrocytes contributes to the hypoxic ventilatory response via a CaThe Journal of physiology · 2018Article
- A genetically encoded CaBMC biology · 2018Article
- Inspiratory-Activated Airway Vagal Preganglionic Neurones Excited by Thyrotropin-Releasing Hormone via Multiple Mechanisms in Neonatal Rats.Frontiers in physiology · 2018Article
- Role of microglial amylin receptors in mediating beta amyloid (Aβ)-induced inflammation.Journal of neuroinflammation · 2017Article
- Article
15 more citing papers are in PubMed but not listed here.
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7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pre-Bötzinger complex (PBC) inspiratory center remains active in a transverse brainstem slice. Such slices are studied at high (8-10 mM) superfusate [K+], which could attenuate the sensitivity of the PBC to neuromodulators such as opiates. Findings may also be confounded because slice boundaries, drug injection sites, or location of rhythmogenic interneurons are rarely verified histologically. Thus, we first generated PBC slices with defined boundaries using novel "on-line histology" based on our finding that rostrocaudal extensions of brainstem respiratory marker nuclei are constant in newborn rats between postnatal days 0-4. At physiological superfusate [K+] (3 mM), 500- and 600-microm-thick slices with the PBC in the center and the caudal boundary 0.70 and 0.76 mm caudal to the facial motonucleus generated rhythm for >2 and approximately 4 h, respectively. Rhythm was abolished by low nanomolar concentrations of the mu-opiate receptor agonist DAMGO ([D-Ala2, N-Me-Phe4, Gly5-ol]enkephalin). After spontaneous arrest of bursting, rhythm was reactivated at clinically relevant or physiological concentrations by 3,5-dihydroxyphenylglycine, thyrotropin-releasing hormone, or rolipram, each affecting distinct second-messenger pathways. Two-photon/confocal Ca2+ imaging revealed that these agents reactivated the same PBC neurons initially active in 3 mM [K+]. The data show that "calibrated" PBC slices at physiological [K+] generate rhythm with a high sensitivity to neuromodulators for extended time periods, whereas spontaneous "in vitro apnea" is an important tool to study the interaction of signaling pathways that modulate rhythm. Our approaches and findings provide the basis for a pharmacological and structure-function analysis of the isolated respiratory center in a histologically well defined substrate at physiological [K+].
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.