ArticleJournal of clinical microbiology2007
Fast DNA serotyping of Escherichia coli by use of an oligonucleotide microarray.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Molecular characterisation of extended-spectrum ß-lactamase producing Escherichia coli in wild birds and cattle, Ibadan, Nigeria.BMC veterinary research · 2021Article
- Development of a Molecular Serotyping Scheme forFrontiers in microbiology · 2021Article
- Genetic Characterization of the O-Antigen and Development of a Molecular Serotyping Scheme forFrontiers in microbiology · 2020Article
- Development of rapid and simple experimental and in silico serotyping systems for Citrobacter.Future microbiology · 2018Article
- Establishment of a Molecular Serotyping Scheme and a Multiplexed Luminex-Based Array forFrontiers in microbiology · 2018Article
- Genetic makeup of Shiga toxin-producing Escherichia coli in relation to clinical symptoms and duration of shedding: a microarray analysis of isolates from Swedish children.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- The Accessory Genome of Shiga Toxin-Producing Escherichia coli Defines a Persistent Colonization Type in Cattle.Applied and environmental microbiology · 2016Article
- Article
- Advances in Molecular Serotyping and Subtyping of Escherichia coli.Frontiers in microbiology · 2016Review
- Comparative Genomics and Characterization of Hybrid Shigatoxigenic and Enterotoxigenic Escherichia coli (STEC/ETEC) Strains.PloS one · 2015Article
- Novel microarray design for molecular serotyping of shiga toxin- producing Escherichia coli strains isolated from fresh produce.Applied and environmental microbiology · 2014Article
- Occurrence of Enterobacteriaceae in Raw Meat and in Human Samples from Egyptian Retail Sellers.International scholarly research notices · 2014Article
- Pathotyping bla CTX-M Escherichia coli from Nigeria.European journal of microbiology & immunology · 2013Article
- Genome sequence of the hemolytic-uremic syndrome-causing strain Escherichia coli NCCP15647.Journal of bacteriology · 2012Article
- Genome sequence of enterohemorrhagic Escherichia coli NCCP15658.Journal of bacteriology · 2012Article
- Zoonotic agents in small ruminants kept on city farms in southern Germany.Applied and environmental microbiology · 2012Article
- Article
- O-antigen and virulence profiling of shiga toxin-producing Escherichia coli by a rapid and cost-effective DNA microarray colorimetric method.Frontiers in cellular and infection microbiology · 2012Article
- Microbial Diagnostic Microarrays for the Detection and Typing of Food- and Water-Borne (Bacterial) Pathogens.Microarrays (Basel, Switzerland) · 2011Review
- Rapid genoserotyping tool for classification of Salmonella serovars.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Classical antibody-based serotyping of Escherichia coli is an important method in diagnostic microbiology for epidemiological purposes, as well as for a rough virulence assessment. However, serotyping is so tedious that its use is restricted to a few reference laboratories. To improve this situation we developed and validated a genetic approach for serotyping based on the microarray technology. The genes encoding the O-antigen flippase (wzx) and the O-antigen polymerase (wzy) were selected as target sequences for the O antigen, whereas fliC and related genes, which code for the flagellar monomer, were chosen as representatives for the H phenotype. Starting with a detailed bioinformatic analysis and oligonucleotide design, an ArrayTube-based assay was established: a fast and robust DNA extraction method was coupled with a site-specific, linear multiplex labeling procedure and hybridization analysis of the biotinylated amplicons. The microarray contained oligonucleotide DNA probes, each in duplicate, representing 24 of the epidemiologically most relevant of the over 180 known O antigens (O antigens 4, 6 to 9, 15, 26, 52, 53, 55, 79, 86, 91, 101, 103, 104, 111, 113, 114, 121, 128, 145, 157, and 172) as well as 47 of the 53 different H antigens (H antigens 1 to 12, 14 to 16, 18 to 21, 23 to 34, 37 to 43, 45, 46, 48, 49, 51 to 54, and 56). Evaluation of the microarray with a set of defined strains representing all O and H serotypes covered revealed that it has a high sensitivity and a high specificity. All of the conventionally typed 24 O groups and all of the 47 H serotypes were correctly identified. Moreover, strains which were nonmotile or nontypeable by previous serotyping assays yielded unequivocal results with the novel ArrayTube assay, which proved to be a valuable alternative to classical serotyping, allowing processing of single colonies within a single working day.
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Registered trials
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