ArticleJournal of virology2007
Identification of a ribonucleoprotein intermediate of tomato mosaic virus RNA replication complex formation.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Long-distance RNA-RNA interactions shape dynamic RNA architectures and functional states in plant positive-strand RNA viruses.Frontiers in microbiology · 2026Review
- A spontaneous thermo-sensitive female sterility mutation in rice enables fully mechanized hybrid breeding.Cell research · 2022Article
- Article
- The Plant Noncanonical Antiviral Resistance Protein JAX1 Inhibits Potexviral Replication by Targeting the Viral RNA-Dependent RNA Polymerase.Journal of virology · 2019Article
- Homeologs of the Nicotiana benthamiana Antiviral ARGONAUTE1 Show Different Susceptibilities to microRNA168-Mediated Control.Plant physiology · 2015Article
- Viruses roll the dice: the stochastic behavior of viral genome molecules accelerates viral adaptation at the cell and tissue levels.PLoS biology · 2015Article
- Replication protein of tobacco mosaic virus cotranslationally binds the 5' untranslated region of genomic RNA to enable viral replication.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- The resistance protein Tm-1 inhibits formation of a Tomato mosaic virus replication protein-host membrane protein complex.Journal of virology · 2013Article
- p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of Tomato bushy stunt virus in yeast cell-free extract.Journal of virology · 2012Article
- Coevolution and hierarchical interactions of Tomato mosaic virus and the resistance gene Tm-1.PLoS pathogens · 2012Article
- A host small GTP-binding protein ARL8 plays crucial roles in tobamovirus RNA replication.PLoS pathogens · 2011Article
- Gaining replicability in a nonhost compromises the silencing suppression activity of Tobacco mild green mosaic virus in a host.Journal of virology · 2011Article
- Template recognition mechanisms by replicase proteins differ between bipartite positive-strand genomic RNAs of a plant virus.Journal of virology · 2011Article
- Inhibition of sterol biosynthesis reduces tombusvirus replication in yeast and plants.Journal of virology · 2010Article
- An inhibitory interaction between viral and cellular proteins underlies the resistance of tomato to nonadapted tobamoviruses.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Mechanism of induction and suppression of antiviral immunity directed by virus-derived small RNAs in Drosophila.Cell host & microbe · 2008Article
- Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast.Journal of virology · 2008Article
- An inhibitor of viral RNA replication is encoded by a plant resistance gene.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The replication of eukaryotic positive-strand RNA virus genomes occurs in the membrane-bound RNA replication complexes. Previously, we found that the extract of evacuolated tobacco BY-2 protoplasts (BYL) is capable of supporting the translation and subsequent replication of the genomic RNAs of plant positive-strand RNA viruses, including Tomato mosaic virus (ToMV). Here, to dissect the process that precedes the formation of ToMV RNA replication complexes, we prepared membrane-depleted BYL (mdBYL), in which the membranes were removed by centrifugation. In mdBYL, ToMV RNA was translated to produce the 130-kDa and 180-kDa replication proteins, but the synthesis of any ToMV-related RNAs did not occur. When BYL membranes were added back to the ToMV RNA-translated mdBYL after the termination of translation with puromycin, ToMV RNA was replicated. Using a replication-competent ToMV derivative that encodes the FLAG-tagged 180-kDa replication protein, it was shown by affinity purification that a complex that contained the 130-kDa and 180-kDa proteins and ToMV genomic RNA was formed after translation in mdBYL. When the complex was mixed with BYL membranes, ToMV RNA was replicated, which suggests that this ribonucleoprotein complex is an intermediate of ToMV RNA replication complex formation. We have named this ribonucleoprotein complex the "pre-membrane-targeting complex." Our data suggest that the formation of the pre-membrane-targeting complex is coupled with the translation of ToMV RNA, while posttranslationally added exogenous 180-kDa protein and replication templates can contribute to replication and can be replicated, respectively. Based on these results, we discuss the mechanisms of ToMV RNA replication complex formation.
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