ArticleWorld journal of gastroenterology2006
Cloning and expression of SLC10A4, a putative organic anion transport protein.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- The Pathological Effects of Circulating Hydrophobic Bile Acids in Alzheimer's Disease.Journal of Alzheimer's disease reports · 2023Review
- SLC10A7, an orphan member of the SLC10 family involved in congenital disorders of glycosylation.Human genetics · 2022Article
- Sigmoid colon mucosal gene expression supports alterations of neuronal signaling in irritable bowel syndrome with constipation.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo.Scientific reports · 2017Article
- NaAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2017Article
- Characterization of cognitive impairments and neurotransmitter changes in a novel transgenic mouse lacking Slc10a4.Neuroscience · 2016Article
- Expression, sorting and transport studies for the orphan carrier SLC10A4 in neuronal and non-neuronal cell lines and in Xenopus laevis oocytes.BMC neuroscience · 2015Article
- Role of the intestinal bile acid transporters in bile acid and drug disposition.Handbook of experimental pharmacology · 2011Review
- The solute carrier family 10 (SLC10): beyond bile acid transport.Molecular aspects of medicineReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
aimTo determine if novel bile acid transporters may be expressed in human tissues.
methodsSLC10A1 (NTCP) was used as a probe to search the NCBI database for homology to previously uncharacterized ESTs. The homology search identified an EST (termed SLC10A4) that shares sequence identity with SLC10A1 and SLC10A2 (ASBT). We performed Northern blot analysis and RT-PCR to determine the tissue distribution of SLC10A4. SLC10A4 was cloned in frame with an epitope tag and overexpressed in CHO cells to determine cellular localization and functional analysis of bile acid uptake.
resultsNorthern analysis revealed that SLC10A4 mRNA is ubiquitously expressed in human tissues with the highest levels of mRNA expression in brain, placenta, and liver. In SLC10A4-transfected CHO cells, immunoblotting analysis and immunofluorescence staining demonstrated a 49-kDa protein that is expressed at the plasma membrane and intracellular compartments. Functional analysis of SLC10A4 showed no significant taurocholate uptake in the presence of sodium when compared to untransfected CHO cells.
conclusionTo date, we have shown that this protein has no capacity to transport taurocholate relative to SLC10A1; however, given its ubiquitous tissue distribution, it may play a more active role in transporting other endogenous organic anions.
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