ArticleJournal of bacteriology1991
Escherichia coli Fpg protein and UvrABC endonuclease repair DNA damage induced by methylene blue plus visible light in vivo and in vitro.
Article in Journal of bacteriology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 101 citations in OpenAlex.
- Dyes in aquaculture and reference points for action.EFSA journal. European Food Safety Authority · 2017Article
- UvrA expression ofJournal of food science and technology · 2017Article
- Insights into the glycosylase search for damage from single-molecule fluorescence microscopy.DNA repair · 2014Review
- Two glycosylase families diffusively scan DNA using a wedge residue to probe for and identify oxidatively damaged bases.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- DNA glycosylases search for and remove oxidized DNA bases.Environmental and molecular mutagenesis · 2013Review
- Important role for Mycobacterium tuberculosis UvrD1 in pathogenesis and persistence apart from its function in nucleotide excision repair.Journal of bacteriology · 2012Article
- The role of Fpg protein in UVC-induced DNA lesions.Redox report : communications in free radical research · 2012Article
- ERCC1 and ERCC2 polymorphisms and adult glioma.Neuro-oncology · 2005Article
- Structural insights into abasic site for Fpg specific binding and catalysis: comparative high-resolution crystallographic studies of Fpg bound to various models of abasic site analogues-containing DNA.Nucleic acids research · 2005Article
- 8-oxo-7,8-dihydroguanine is removed by a nucleotide excision repair-like mechanism in Porphyromonas gingivalis W83.Journal of bacteriology · 2004Article
- Review
- Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA.The EMBO journal · 2002Article
- DNA repair and sequence context affect (1)O(2)-induced mutagenesis in bacteria.Nucleic acids research · 2001Article
- Repair of DNA lesions induced by hydrogen peroxide in the presence of iron chelators in Escherichia coli: participation of endonuclease IV and Fpg.Journal of bacteriology · 2000Article
- DNA polymerase II (polB) is involved in a new DNA repair pathway for DNA interstrand cross-links in Escherichia coli.Journal of bacteriology · 1999Article
- Efficient in vitro repair of 7-hydro-8-oxodeoxyguanosine by human cell extracts: involvement of multiple pathways.Nucleic acids research · 1998Article
- Protection of DNA during oxidative stress by the nonspecific DNA-binding protein Dps.Journal of bacteriology · 1997Article
- Cancer risk and oxidative DNA damage in man.Journal of molecular medicine (Berlin, Germany) · 1996Review
- Substrate spectrum of human excinuclease: repair of abasic sites, methylated bases, mismatches, and bulky adducts.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Formamidopyrimidine DNA glycosylase in the yeast Saccharomyces cerevisiae.Nucleic acids research · 1994Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
pBR322 plasmid DNA was treated with methylene blue plus visible light (MB-light) and tested for transformation efficiency in Escherichia coli mutants defective in either formamidopyrimidine-DNA glycosylase (Fpg protein) and/or UvrABC endonuclease. The survival of pBR322 DNA treated with MB-light was not significantly reduced when transformed into either fpg-1 or uvrA single mutants compared with that in the wild-type strain. In contrast, the survival of MB-light-treated pBR322 DNA was greatly reduced in the fpg-1 uvrA double mutant. The synergistic effect of these two mutations was not observed in transformation experiments using pBR322 DNA treated with methyl methanesulfonate, UV light at 254 nm, or ionizing radiation. In vitro experiments showed that MB-light-treated pBR322 DNA is a substrate for the Fpg protein and UvrABC endonuclease. The number of sites sensitive to cleavage by either Fpg protein or UvrABC endonuclease was 10-fold greater than the number of apurinic-apyrimidinic sites indicated as Nfo protein (endonuclease IR)-sensitive sites. Seven Fpg protein-sensitive sites per PBR322 molecule were required to produce a lethal hit when transformed into the uvrA fpg-1 mutant. These results suggest that MB-light induces DNA base modifications which are lethal and that these modifications are repaired by Fpg protein and UvrABC endonuclease in vivo and in vitro. Therefore, one of the physiological functions of Fpg protein might be to repair DNA base damage induced by photosensitizers and light.
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