ArticleProceedings of the National Academy of Sciences of the United States of America2006
Murine cytomegalovirus encodes a stable intron that facilitates persistent replication in the mouse.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 49 citations in OpenAlex.
- Characterization of Human Cytomegalovirus (HCMV) Long Non-Coding RNA1.2 During Lytic Replication.Viruses · 2025Article
- Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers.PLoS genetics · 2024Article
- A 44-Nucleotide Region in the Chikungunya Virus 3' UTR Dictates Viral Fitness in Disparate Host Cells.Viruses · 2024Article
- Decoding murine cytomegalovirus.PLoS pathogens · 2023Article
- Article
- Idiosyncrasies of Viral Noncoding RNAs Provide Insights into Host Cell Biology.Annual review of virology · 2019Review
- Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus.Virology · 2019Article
- Expression of pseudorabies virus-encoded long noncoding RNAs in epithelial cells and neurons.Journal of neurovirology · 2018Article
- ShapeShifter: a novel approach for identifying and quantifying stable lariat intronic species in RNAseq data.Quantitative biology (Beijing, China) · 2018Article
- On the Demographic and Selective Forces Shaping Patterns of Human Cytomegalovirus Variation within Hosts.Pathogens (Basel, Switzerland) · 2018Review
- Long Non-Coding RNAs: Novel Players in Regulation of Immune Response Upon Herpesvirus Infection.Frontiers in immunology · 2018Review
- Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences.Molecular therapy. Nucleic acids · 2017Article
- Roles of LncRNAs in Viral Infections.Frontiers in cellular and infection microbiology · 2017Review
- Stable intronic sequence RNAs (sisRNAs): a new layer of gene regulation.Cellular and molecular life sciences : CMLS · 2016Review
- Functional Dissection of an Alternatively Spliced Herpesvirus Gene by Splice Site Mutagenesis.Journal of virology · 2016Article
- Long noncoding RNAs in innate immunity.Cellular & molecular immunology · 2016Review
- On the relative roles of background selection and genetic hitchhiking in shaping human cytomegalovirus genetic diversity.Molecular ecology · 2016Article
- Viral noncoding RNAs: more surprises.Genes & development · 2015Review
- EBV Noncoding RNAs.Current topics in microbiology and immunology · 2015Review
- Stability determinants of murine cytomegalovirus long noncoding RNA7.2.Journal of virology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The human cytomegalovirus immediate-early 5-kb RNA previously has been shown to be a stable intron that is not required for efficient replication of the virus in cultured fibroblasts. Here we describe a murine cytomegalovirus 7.2-kb ortholog of the human cytomegalovirus 5-kb RNA. The 5' end of the 7.2-kb transcript maps to a consensus splice-donor site that is conserved among all cytomegaloviruses. We constructed mutant viruses lacking the entire 7.2-kb coding domain, the splice-donor site predicted to function in the generation of the intron or a hairpin predicted to stabilize the intron. All three mutant viruses failed to produce the 7.2-kb RNA, supporting our conclusion that it is a stable intron. Each of the mutants replicated with normal kinetics in cultured fibroblasts, but the mutants exhibited a clear defect within infected mice. Although the initial acute phase at 4 days after infection appeared to be normal, none of the mutant viruses progressed to the persistent phase, i.e., little virus was detected in the salivary gland at 14 days after infection. The intron functions as an in vivo virulence factor, facilitating progression from the acute to persistent phase of infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.