ArticleTransgenic research2007
Knockdown of berberine bridge enzyme by RNAi accumulates (S)-reticuline and activates a silent pathway in cultured California poppy cells.
Article in Transgenic research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 111 citations in OpenAlex.
- Genome-wide transcriptional and metabolic responses of Eschscholzia californica to salt stress.BMC plant biology · 2026Article
- Metabolomic Insights into Sexual Multi-Morphism of Sinomenine Accumulation inPlants (Basel, Switzerland) · 2025Article
- Herbgenomics meets Papaveraceae: a promising -omics perspective on medicinal plant research.Briefings in functional genomics · 2024Review
- A cornucopia of diversity-Ranunculales as a model lineage.Journal of experimental botany · 2024Review
- Article
- California poppy (Frontiers in plant science · 2023Review
- Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.Molecules (Basel, Switzerland) · 2022Review
- Isolation and characterization of twoThe Journal of biological chemistry · 2020Article
- Molecular Origins of Functional Diversity in Benzylisoquinoline Alkaloid Methyltransferases.Frontiers in plant science · 2019Review
- Metabolic engineering for the production of plant isoquinoline alkaloids.Plant biotechnology journal · 2016Review
- Efficient microbial production of stylopine using a Pichia pastoris expression system.Scientific reports · 2016Article
- Changing trends in biotechnology of secondary metabolism in medicinal and aromatic plants.Planta · 2015Article
- Non-coding RNAs in crop genetic modification: considerations and predictable environmental risk assessments (ERA).Molecular biotechnology · 2013Review
- MicroRNA-mediated gene regulation: potential applications for plant genetic engineering.Plant molecular biology · 2013Review
- Recent progress in the metabolic engineering of alkaloids in plant systems.Current opinion in biotechnology · 2013Review
- Microbial production of isoquinoline alkaloids as plant secondary metabolites based on metabolic engineering research.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2013Review
- Engineering microbial factories for synthesis of value-added products.Journal of industrial microbiology & biotechnology · 2011Review
- Article
- Role of RNA interference in plant improvement.Die Naturwissenschaften · 2011Review
- Microbial production of plant benzylisoquinoline alkaloids.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reticuline is a key compound in the biosynthetic pathway for isoquinoline alkaloids in plants, which include morphine, codeine and berberine. We established cultured California poppy (Eschscholzia californica) cells, in which berberine bridge enzyme (BBE) was knocked down by RNA interference, to accumulate the important key intermediate reticuline. Both BBE mRNA accumulation and enzyme activity were effectively suppressed in transgenic cells. In these transgenic cells, end-products of isoquinoline alkaloid biosynthesis, such as sanguinarine, were considerably reduced and reticuline was accumulated at a maximum level of 310 mug/g-fresh weight. In addition, 1 g-fresh weight of these cells secreted significant amounts of reticuline into the medium, with a maximum level of 6 mg/20 mL culture medium. These cells also produced a methylated derivative of reticuline, laudanine, which could scarcely be detected in control cells. We discuss the potential application of RNAi technology in metabolic modification and the flexibility of plant secondary metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.