ArticleTransgenic research2007
Production of siRNA targeted against TYLCV coat protein transcripts leads to silencing of its expression and resistance to the virus.
Article in Transgenic research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 89 citations in OpenAlex.
- In Silico Investigation of the Interactions Between Cotton Leaf Curl Multan Virus Proteins and the Transcriptional Gene Silencing Factors of Gossypium hirsutum L.Journal of molecular evolution · 2024Article
- Development, Design, and Application of Efficient siRNAs Against Cotton Leaf Curl Virus-Betasatellite Complex to Mediate Resistance Against Cotton Leaf Curl Disease.Indian journal of microbiology · 2024Article
- Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato.Plants (Basel, Switzerland) · 2023Article
- RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi.International journal of molecular sciences · 2023Review
- Development of a method to rapidly assess resistance/susceptibility of Micro-Tom tomatoes to Tomato yellow leaf curl virus via agroinoculation of cotyledons.BMC research notes · 2021Article
- Biotic stress triggered small RNA and RNAi defense response in plants.Molecular biology reports · 2020Review
- Differential responses ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Review
- Plant Small Non-coding RNAs and Their Roles in Biotic Stresses.Frontiers in plant science · 2018Review
- Genetically modified (GM) crops: milestones and new advances in crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Review
- Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection.BMC plant biology · 2016Article
- RNAi mediated broad-spectrum transgenic resistance in Nicotiana benthamiana to chilli-infecting begomoviruses.Plant cell reports · 2015Article
- Application of molecular antiviral compounds: novel approach for durable resistance against geminiviruses.Molecular biology reports · 2015Review
- Article
- Identification of siRNA generating hot spots in multiple viral suppressors to generate broad-spectrum antiviral resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- Artificial microRNA mediated gene silencing in plants: progress and perspectives.Plant molecular biology · 2014Review
- Generation of marker-free transgenic plants concurrently resistant to a DNA geminivirus and a RNA tospovirus.Scientific reports · 2014Article
- Optimisation of tomato Micro-tom regeneration and selection on glufosinate/Basta and dependency of gene silencing on transgene copy number.Plant cell reports · 2013Article
- Article
- Recruitment of the host plant heat shock protein 70 by Tomato yellow leaf curl virus coat protein is required for virus infection.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The coat protein (CP) of Tomato yellow leaf curl virus (TYLCV), encoded by the v1 gene, is the only known component of the viral capsid. In addition, the CP plays a role in the virus transport into the host cell nucleus where viral genes are replicated and transcribed. In this study, we analyzed the effect of small interfering double-stranded RNAs (siRNAs), derived from an intron-hairpin RNA (ihpRNA) construct and targeting the v1 gene product, on CP accumulation. Transient assays involving agroinfiltration of the CP-silencing construct followed by infiltration of a fused GFP-CP (green fluorescent protein-coat protein) gene showed down-regulation of GFP expression in Nicotiana benthamiana. Some of the transgenic tomato plants (cv. Micro-Tom), expressing the siRNA targeted against the TYLCV CP gene, did not show disease symptoms 7 weeks post-inoculation with the virus, while non-transgenic control plants were infected within 2 weeks post inoculation. The present study demonstrates, for the first time, that siRNA targeted against the CP of TYLCV can confer resistance to the virus in transgenic tomato plants, thereby enabling flowering and fruit production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.