ArticleChromosoma2007
Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin.
Article in Chromosoma, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 63 citations in OpenAlex.
- Zygosity-based sex determination in a butterfly drives hypervariability ofScience advances · 2024Article
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- Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae).Scientific reports · 2023Article
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- Article
- Large-scale comparative analysis of cytogenetic markers across Lepidoptera.Scientific reports · 2021Article
- InSexBase: an annotated genomic resource of sex chromosomes and sex-biased genes in insects.Database : the journal of biological databases and curation · 2021Article
- The Role of Satellite DNAs in Genome Architecture and Sex Chromosome Evolution in Crambidae Moths.Frontiers in genetics · 2021Article
- Evolution of multiple sex-chromosomes associated with dynamic genome reshuffling in Leptidea wood-white butterflies.Heredity · 2020Article
- Patterns of Sex Chromosome Differentiation in Spiders: Insights from Comparative Genomic Hybridisation.Genes · 2020Article
- Characterization of the First W-Specific Protein-Coding Gene for Sex Identification inFrontiers in genetics · 2020Article
- Advances and Challenges of Using the Sterile Insect Technique for the Management of Pest Lepidoptera.Insects · 2019Review
- Silkworm genetic sexing through W chromosome-linked, targeted gene integration.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Insights into the Structure of the Spruce Budworm (G3 (Bethesda, Md.) · 2018Article
- Sex Chromosomes of the Iconic MothGenes · 2018Article
- The deep conservation of the Lepidoptera Z chromosome suggests a non-canonical origin of the W.Nature communications · 2017Article
- Neo-sex Chromosomes in the Monarch Butterfly,G3 (Bethesda, Md.) · 2017Article
- W-enriched satellite sequence in the Indian meal moth, Plodia interpunctella (Lepidoptera, Pyralidae).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Article
- Conserved Patterns of Sex Chromosome Dosage Compensation in the Lepidoptera (WZ/ZZ): Insights from a Moth Neo-Z Chromosome.Genome biology and evolution · 2017Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and used to prepare painting probes and start an analysis of the W-chromosome sequence composition. With fluorescence in situ hybridization (FISH), the euchromatin probe labelled all chromosomes, whereas the W-chromatin DNA proved to be a highly specific W-chromosome painting probe. For sequence analysis, DOP-PCR-generated DNA fragments were cloned, sequenced, and tested by Southern hybridization. We recovered single-copy and low-copy W-specific sequences, a sequence that was located only in the W and the Z chromosome, multi-copy sequences that were enriched in the W chromosome but occurred also elsewhere, and ubiquitous multi-copy sequences. Three of the multi-copy sequences were recognized as derived from hitherto unknown retrotransposons. The results show that our approach is feasible and that the W-chromosome composition of C. pomonella is not principally different from that of Bombyx mori or from that of Y chromosomes of several species with an XY sex-determining mechanism. The W chromosome has attracted repetitive sequences during evolution but also contains unique sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.