ArticlePlant cell reports2007
Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 116 citations in OpenAlex.
- Solanum villosum Mill. an underutilized plant: establishment of hairy root culture and enhanced production of solasodine after elicitor treatment.Protoplasma · 2025Article
- Agrobacterium-Mediated Genetic Transformation in Ashwagandha (Withania somnifera).Methods in molecular biology (Clifton, N.J.) · 2025Article
- Agrobacterium-Mediated Genetic Transformation ofACS omega · 2023Article
- Pharmacokinetic Study of Withanosides and Withanolides fromMolecules (Basel, Switzerland) · 2022Article
- Promising prospective effects ofFrontiers in veterinary science · 2022Review
- Review
- Addressing Challenges to Enhance the Bioactives ofBioMed research international · 2017Review
- Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment.3 Biotech · 2015Article
- The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Use of the cryptogein gene to stimulate the accumulation of Bacopa saponins in transgenic Bacopa monnieri plants.Plant cell reports · 2012Article
- Striga parasitizes transgenic hairy roots of Zea mays and provides a tool for studying plant-plant interactions.Plant methods · 2012Article
- Agrobacterium rhizogenes-dependent production of transformed roots from foliar explants of pepper (Capsicum annuum): a new and efficient tool for functional analysis of genes.Plant cell reports · 2012Article
- Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants.Plant cell reports · 2011Article
- Agrobacterium tumefaciens-mediated transformation of Withania somnifera (L.) Dunal: an important medicinal plant.Plant cell reports · 2010Article
- Steroidal lactones from Withania somnifera, an ancient plant for novel medicine.Molecules (Basel, Switzerland) · 2009Review
- Transgenic mimicry of pathogen attack stimulates growth and secondary metabolite accumulation.Transgenic research · 2009Article
Corrections and comments
- Expression of concern
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developmental variability was introduced into Withania somnifera using genetic transformation by Agrobacterium rhizogenes, with the aim of changing withasteroid production. Inoculation of W. somnifera with A. rhizogenes strains LBA 9402 and A4 produced typical transformed root lines, transformed callus lines, and rooty callus lines with simultaneous root dedifferentiation and redifferentiation. These morphologically distinct transformed lines varied in T-DNA content, growth rates, and withasteroid accumulation. All of the lines with the typical transformed root morphology contained the T(L) T-DNA, and 90% of them carried the T(R) T-DNA, irrespective of the strain used for infection. Accumulation of withaferin A was maximum (0.44% dry weight) in the transformed root line WSKHRL-1. This is the first detection of withaferin A in the roots of W. somnifera. All of the rooty callus lines induced by strain A4 contained both the T(L) and the T(R)-DNAs. In contrast, 50% of the rooty-callus lines obtained with strain LBA 9402 contained only the T(R) T-DNA. All the rooty callus lines accumulated both withaferin A and withanolide D. The callusing lines induced by LBA 9402 lacked the T(L) T-DNA genes, while all the callusing lines induced by strain A4 contained the T(L) DNA. Four of these callus lines produced both withaferin A (0.15-0.21% dry weight) and withanolide D (0.08-0.11% dry weight), and they grew faster than the transformed root lines. This is the first report of the presence of withasteroids in undifferentiated callus cultures of W. somnifera.
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