ArticlePlant molecular biology2007
Different phosphorylation mechanisms are involved in the activation of sucrose non-fermenting 1 related protein kinases 2 by osmotic stresses and abscisic acid.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers, 2 of them syntheses that pooled it.
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Who cites it
107 citing papers in PubMed, 2 syntheses or guidelines pooled it, 238 citations in OpenAlex.
- Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition.Frontiers in plant science · 2023Pooled it
- Identification of key genes and its chromosome regions linked to drought responses in leaves across different crops through meta-analysis of RNA-Seq data.BMC plant biology · 2019Pooled it
- The U-box E3 ubiquitin ligase PUB35 negatively regulates ABA signaling through AFP1-mediated degradation of ABI5.The Plant cell · 2024Article
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- Unveiling the crucial roles of abscisic acid in plant physiology: implications for enhancing stress tolerance and productivity.Frontiers in plant science · 2024Review
- The osmotic stress-activated receptor-like kinase DPY1 mediates SnRK2 kinase activation and drought tolerance in Setaria.The Plant cell · 2023Article
- Constitutively active B2 Raf-like kinases are required for drought-responsive gene expression upstream of ABA-activated SnRK2 kinases.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Identification ofPlants (Basel, Switzerland) · 2023Article
- Genome-wide investigation of SnRK2 gene family in two jute species: Corchorus olitorius and Corchorus capsularis.Journal, genetic engineering & biotechnology · 2023Article
- Plant salinity stress, sensing, and its mitigation through WRKY.Frontiers in plant science · 2023Review
- Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.BMC genomics · 2022Article
- Plant hormone regulation of abiotic stress responses.Nature reviews. Molecular cell biology · 2022Review
- Identification and expression analysis of the CqSnRK2 gene family and a functional study of the CqSnRK2.12 gene in quinoa (Chenopodium quinoa Willd.).BMC genomics · 2022Article
- Functional genomics in plant abiotic stress responses and tolerance: From gene discovery to complex regulatory networks and their application in breeding.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Review
- Mining the Roles of Wheat (Frontiers in plant science · 2022Article
- Genome-Wide Identification and Characterization of theFrontiers in plant science · 2022Article
- Raf-like kinases and receptor-like (pseudo)kinase GHR1 are required for stomatal vapor pressure difference response.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- GhWRKY21 regulates ABA-mediated drought tolerance by fine-tuning the expression of GhHAB in cotton.Plant cell reports · 2021Article
- The Multifaceted Regulation of SnRK2 Kinases.Cells · 2021Review
47 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Arabidopsis cell suspension, hyperosmotic stresses (mannitol and NaCl) were previously shown to activate nine sucrose non-fermenting 1 related protein kinases 2 (SnRK2s) whereas only five of them were also activated by abscisic acid (ABA) treatment. Here, the possible activation by phosphorylation/ dephosphorylation of each kinase was investigated by studying their phosphorylation state after osmotic stress, using the Pro-Q Diamond, a specific dye for phosphoproteins. All the activated kinases were phosphorylated after osmotic stress but the induced phosphorylation changes were clearly different depending on the kinase. In addition, the increase of the global phosphorylation level induced by ABA application was lower, suggesting that different mechanisms may be involved in SnRK2 activation by hyperosmolarity and ABA. On the other hand, SnRK2 kinases remain activated by hyperosmotic stress in ABA-deficient and ABA-insensitive mutants, indicating that SnRK2 osmotic activation is independent of ABA. Moreover, using a mutant form of SnRK2s, a specific serine in the activation loop was shown to be phosphorylated after stress treatments and essential for activity and/or activation. Finally, SnRK2 activity was sensitive to staurosporine, whereas SnRK2 activation by hyperosmolarity or ABA was not, indicating that SnRK2 activation by phosphorylation is mediated by an upstream staurosporine-insensitive kinase, in both signalling pathways. All together, these results indicate that different phosphorylation mechanisms and at least three signalling pathways are involved in the activation of SnRK2 proteins in response to osmotic stress and ABA.
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