ArticlePlant cell reports2007
Co-bombardment, integration and expression of rice chitinase and thaumatin-like protein genes in barley (Hordeum vulgare cv. Conlon).
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 39 citations in OpenAlex.
- Impact of wheat GRF4-GIF1 morphogenic regulators on transformation and genome editing efficiency in elite barley cultivars.Frontiers in plant science · 2026Article
- Selection of stable reference genes in prunus persica fruit infected with monilinia laxa for normalisation of RT-qPCR gene expression data.Scientific reports · 2025Article
- Towards Biological Control ofPlants (Basel, Switzerland) · 2021Article
- Overexpression of rice thaumatin-like protein (Journal, genetic engineering & biotechnology · 2018Article
- Effect of antibiotics on callus regeneration during transformation of IR 64 rice.Biotechnology reports (Amsterdam, Netherlands) · 2015Article
- Marker-free transgenic corn plant production through co-bombardment.Plant cell reports · 2009Article
- Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants.Plant cell reports · 2009Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogenesis-related (PR) proteins associated with degradation of structural components of pathogenic filamentous fungi were overexpressed in the two-rowed malting barley (Hordeum vulgare L.) cultivar Conlon. Transgenes were introduced by co-bombardment with two plasmids, one carrying a rice (Oryza sativa L.) chitinase gene (chi11) and another carrying a rice thaumatin-like protein gene (tlp). Each gene was under the control of the maize ubiquitin (Ubi1) promoter. Fifty-eight primary transformants from three independent transformation events were regenerated. T(1) plants with high rice chi11 and tlp protein expression levels were advanced to identify T(2) homozygotes by herbicide spray and subjected to further molecular analyses. T(3) progeny from one event (E2) had stable integration and expression of the rice chi11 and tlp while those from the other events (E1 and E3) showed stable integration only of tlp. The successful production of these lines overexpressing the antifungal chi and tlp proteins provides materials to test the effects of these genes on a variety of fungal diseases that attack barley and to serve as potential additional sources of disease resistance.
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