ArticleHistochemistry and cell biology2007
High-efficiency gene transfer into cultured embryonic motoneurons using recombinant lentiviruses.
Article in Histochemistry and cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 18 citations in OpenAlex.
- Tropism, intracerebral distribution, and transduction efficiency of HIV- and SIV-based lentiviral vectors after injection into the mouse brain: a qualitative and quantitative in vivo study.Histochemistry and cell biology · 2017Article
- Biomedical techniques in translational studies: The journey so far.Nigerian medical journal : journal of the Nigeria Medical Association · 2014Article
- Multisite phosphorylation of c-Jun at threonine 91/93/95 triggers the onset of c-Jun pro-apoptotic activity in cerebellar granule neurons.Cell death & disease · 2013Article
- Article
- Local axonal function of STAT3 rescues axon degeneration in the pmn model of motoneuron disease.The Journal of cell biology · 2012Article
- The critical role of CD133(+)CD44(+/high) tumor cells in hematogenous metastasis of liver cancers.Cell research · 2012Article
- Cortical overexpression of neuronal calcium sensor-1 induces functional plasticity in spinal cord following unilateral pyramidal tract injury in rat.PLoS biology · 2010Article
- High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function.Molecular neurodegeneration · 2010Article
- Functional protein delivery into neurons using polymeric nanoparticles.The Journal of biological chemistry · 2009Article
- Novel role for vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Recent progress in histochemistry.Histochemistry and cell biology · 2007Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primary neurons are a common tool for investigating gene function for survival and morphological and functional differentiation. Gene transfer techniques play an important role in this context. However, the efficacy of conventional gene transfer techniques, in particular for primary motoneurons is low so that it is not possible to distinguish whether the observed effects are representative for all neurons or only for the small subpopulation that expresses the transfected cDNA. In order to develop techniques that allow high gene transfer rates, we have optimized lentiviral-based gene transfer for cultured motoneurons by using a replication-defective viral vector system. These techniques result in transduction efficacies higher than 50%, as judged by EGFP expression under the control of SFFV or CMV promoters. Under the same conditions, survival and morphology of the cultured motoneurons was not altered, at least not when virus titers did not exceed a multiplicity of infection of 100. Under the same cell culture conditions, electroporation resulted in less than 5% transfected motoneurons and reduced survival. Therefore we consider this lentivirus-based gene transfer protocol as a suitable tool to study the effects of gene transfer on motoneuron survival, differentiation and function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.