ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2007
Identification of AFLP markers linked to the male fertility restorer gene of CMS (Moricandia arvensis) Brassica juncea and conversion to SCAR marker.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- A pentatricopeptide repeat protein restores fertility in Moricandia arvensis based cytoplasmic male sterility system in Brassica juncea.Molecular genetics and genomics : MGG · 2025Article
- Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops.International journal of molecular sciences · 2022Review
- Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding.Plant reproduction · 2019Review
- Mitochondrial genome and transcriptome analysis of five alloplasmic male-sterile lines in Brassica juncea.BMC genomics · 2019Article
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Authors and funding
4 authors.
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Abstract
We have developed a cytoplasmic male sterile (CMS) line of Brassica juncea through somatic hybridization with Moricandia arvensis and introgressed the fertility restorer gene into B. juncea. This fertility restorer locus is unique in that it is capable of restoring male fertility to two other alloplasmic CMS systems of B. juncea. As a first step toward cloning of this restorer gene we attempted molecular tagging of the Rf locus using the amplified fragment length polymorphism (AFLP) technique. A BC(1)F(1) population segregating for male sterility/fertility was used for tagging using the bulk segregant analysis method. Out of 64 primer combinations tested in the bulks, 5 combinations gave polymorphic amplification patterns. Further testing of these primers in individual plants showed four amplicons associated with the male fertility trait. Polymorphic amplicons were cloned and used for designing SCAR primers. One of the SCAR primers generated amplicons mostly in the fertile plants. Linkage analysis using MAPMAKER showed two AFLP and one SCAR markers linked to the male fertility gene with a map distance ranging from 0.6 to 2.9 cM. All the markers are located on one side of the Rf locus.
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