ArticleMolecular and cellular biology2007
The doublesex splicing enhancer components Tra2 and Rbp1 also repress splicing through an intronic silencer.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Molecular characterization of transformer, transformer-2, and doublesex genes in the carambola fruit fly, Bactrocera carambolae, revealing potential for genetic pest management.Insect science · 2026Article
- Isolation and Molecular Characterization of the Transformer Gene From Bactrocera cucurbitae (Diptera: Tephritidae).Journal of insect science (Online) · 2017Article
- The study of the transformer gene from Bactrocera dorsalis and B. correcta with putative core promoter regions.BMC genetics · 2016Article
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- A novel splicing silencer generated by DMD exon 45 deletion junction could explain upstream exon 44 skipping that modifies dystrophinopathy.Journal of human genetics · 2014Article
- Tribolium castaneum Transformer-2 regulates sex determination and development in both males and females.Insect biochemistry and molecular biology · 2013Article
- Half pint/Puf68 is required for negative regulation of splicing by the SR splicing factor Transformer2.RNA biology · 2013Article
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- Genetic variation in the Yolk protein expression network of Drosophila melanogaster: sex-biased negative correlations with longevity.Heredity · 2012Article
- Activation and repression functions of an SR splicing regulator depend on exonic versus intronic-binding position.Nucleic acids research · 2012Article
- Genomic organization and splicing evolution of the doublesex gene, a Drosophila regulator of sexual differentiation, in the dengue and yellow fever mosquito Aedes aegypti.BMC evolutionary biology · 2011Article
- The gene transformer-2 of Anastrepha fruit flies (Diptera, Tephritidae) and its evolution in insects.BMC evolutionary biology · 2010Article
- DX16 is a novel SR protein phosphorylated by DOA.Molecular and cellular biochemistry · 2008Article
- The gene transformer of anastrepha fruit flies (Diptera, tephritidae) and its evolution in insects.PloS one · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The activation of sex-specific alternative splice sites in the Drosophila melanogaster doublesex and fruitless pre-mRNAs has been well studied and depends on the serine-arginine-rich (SR) splicing factors Tra, Tra2, and Rbp1. Little is known, however, about how SR factors negatively regulate splice sites in other RNAs. Here we examine how Tra2 blocks splicing of the M1 intron from its own transcript. We identify an intronic splicing silencer (ISS) adjacent to the M1 branch point that is sufficient to confer Tra2-dependent repression on another RNA. The ISS was found to function independently of its position within the intron, arguing against the idea that bound repressors function by simply interfering with branch point accessibility to general splicing factors. Conserved subelements of the silencer include five short repeated sequences that are required for Tra2 binding but differ from repeated binding sites found in Tra2-dependent splicing enhancers. The ISS also contains a consensus binding site for Rbp1, and this protein was found to facilitate repression of M1 splicing both in vitro and in Drosophila larvae. In contrast to the cooperative binding of SR proteins observed on the doublesex splicing enhancer, we found that Rbp1 and Tra2 bind to the ISS independently through distinct sequences. Our results suggest that functionally synergistic interactions of these SR factors can cause either splicing activation or repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.