ArticleMolecular and cellular biology2007
Role of transcription factor Kar4 in regulating downstream events in the Saccharomyces cerevisiae pheromone response pathway.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Engineered Optogenetic Circuits In Yeast with Self-Sustained Outputs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Kar4 acts as a Ste12 regulator inbioRxiv : the preprint server for biology · 2025Article
- Basal association of a transcription factor favors early gene expression.PLoS genetics · 2025Article
- Regulation of meiotic gene expression is functional in the human fungal pathogen Candida glabrata.FEMS yeast research · 2025Article
- Kar4, the yeast homolog of METTL14, is required for mRNA m6A methylation and meiosis.PLoS genetics · 2023Article
- Kar4 is required for the normal pattern of meiotic gene expression.PLoS genetics · 2023Article
- Article
- The protein methylation network in yeast: A landmark in completeness for a eukaryotic post-translational modification.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Vir1p, the yeast homolog of virilizer, is required for mRNA m6A methylation and meiosis.Genetics · 2023Article
- Vir1p, the Yeast Homolog of Virilizer, is Required for mRNA mbioRxiv : the preprint server for biology · 2023Article
- Kar4 is Required for the Normal Pattern of Meiotic Gene Expression.bioRxiv : the preprint server for biology · 2023Article
- Kar4, the Yeast Homolog of METTL14, is Required for mRNA mbioRxiv : the preprint server for biology · 2023Article
- Sporulation inJournal of fungi (Basel, Switzerland) · 2020Review
- NFrontiers in oncology · 2019Review
- Timing of gene expression in a cell-fate decision system.Molecular systems biology · 2018Article
- Article
- A fly view on the roles and mechanisms of the mRNA biology · 2017Article
- Identification of factors required for mThe New phytologist · 2017Article
- Spatial and temporal signal processing and decision making by MAPK pathways.The Journal of cell biology · 2017Review
- Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters.Nature communications · 2016Article
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4 authors.
Funding
Abstract
Yeast Kar4 is a putative transcription factor required for karyogamy (the fusion of haploid nuclei during mating) and possibly other functions. Previously known to be required only for the transcriptional induction of KAR3 and CIK1, microarray experiments identified many genes regulated by Kar4 in both mating and mitosis. Several gene clusters are positively or negatively regulated by mating pheromone in a Kar4-dependent manner. Chromatin immunoprecipitation and gel shift assays confirmed that Kar4 binds to regulatory DNA sequences upstream of KAR3. Together with one-hybrid experiments, these data support a model in which both Kar4 and Ste12 bind jointly to the KAR3 promoter. Analysis of the upstream regions of Kar4-induced genes identified a DNA sequence motif that may be a binding site for Kar4. Mutation within the motif upstream of KAR3 eliminated pheromone induction. Genes regulated by Kar4, on average, are delayed in their temporal expression and exhibit a more stringent dose response to pheromone. Furthermore, the induction of Kar4 by pheromone is necessary for the delayed temporal induction of KAR3 and PRM2, genes required for efficient nuclear fusion during mating. Accordingly, we propose that Kar4 plays a critical role in the choreography of the mating response.
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