ArticleMolecular and cellular biology2007
A novel plasmid-based microarray screen identifies suppressors of rrp6Delta in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- Tunicamycin Sensitivity-Suppression by High Gene Dosage Reveals New Functions of the Yeast Hog1 MAP Kinase.Cells · 2019Article
- The Rrp6 C-terminal domain binds RNA and activates the nuclear RNA exosome.Nucleic acids research · 2017Article
- Control of Saccharomyces cerevisiae pre-tRNA processing by environmental conditions.RNA (New York, N.Y.) · 2016Article
- tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6.RNA (New York, N.Y.) · 2014Article
- Genetic interactions suggest multiple distinct roles of the arch and core helicase domains of Mtr4 in Rrp6 and exosome function.Nucleic acids research · 2013Article
- Novel interactions at the essential N-terminus of poly(A) polymerase that could regulate poly(A) addition in Saccharomyces cerevisiae.FEBS letters · 2012Article
- Interdependent nucleocytoplasmic trafficking and interactions of Dis3 with Rrp6, the core exosome and importin-alpha3.Traffic (Copenhagen, Denmark) · 2009Article
- Core exosome-independent roles for Rrp6 in cell cycle progression.Molecular biology of the cell · 2009Article
- Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants.RNA (New York, N.Y.) · 2008Article
- Covert genetic selections to optimize phenotypes.PloS one · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Genetic screens in Saccharomyces cerevisiae provide novel information about interacting genes and pathways. We screened for high-copy-number suppressors of a strain with the gene encoding the nuclear exosome component Rrp6p deleted, with either a traditional plate screen for suppressors of rrp6Delta temperature sensitivity or a novel microarray enhancer/suppressor screening (MES) strategy. MES combines DNA microarray technology with high-copy-number plasmid expression in liquid media. The plate screen and MES identified overlapping, but also different, suppressor genes. Only MES identified the novel mRNP protein Nab6p and the tRNA transporter Los1p, which could not have been identified in a traditional plate screen; both genes are toxic when overexpressed in rrp6Delta strains at 37 degrees C. Nab6p binds poly(A)+ RNA, and the functions of Nab6p and Los1p suggest that mRNA metabolism and/or protein synthesis are growth rate limiting in rrp6Delta strains. Microarray analyses of gene expression in rrp6Delta strains and a number of suppressor strains support this hypothesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.