ArticleInfection and immunity2007
Cell wall targeting of laccase of Cryptococcus neoformans during infection of mice.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
- Genomic and phenotypic insights into the expanding phylogenetic landscape of the Cryptococcus genus.PLoS genetics · 2025Article
- Genomic and phenotypic insights into the expanding phylogenetic landscape of thebioRxiv : the preprint server for biology · 2025Article
- Mechanisms and Virulence Factors ofJournal of fungi (Basel, Switzerland) · 2024Review
- Glutathione-mediated redox regulation in Cryptococcus neoformans impacts virulence.Nature microbiology · 2024Article
- Reciprocal modulation of ammonia and melanin production has implications for cryptococcal virulence.Nature communications · 2023Article
- Role of the ESCRT Pathway in Laccase Trafficking and Virulence of Cryptococcus neoformans.Infection and immunity · 2020Article
- Contribution of Laccase Expression to Immune Response against Cryptococcus gattii Infection.Infection and immunity · 2020Article
- The structural unit of melanin in the cell wall of the fungal pathogenThe Journal of biological chemistry · 2019Article
- Cell Wall-Associated Virulence Factors Contribute to Increased Resilience of OldFrontiers in microbiology · 2019Article
- IPVirulence · 2017Article
- High-Resolution Genetics Identifies the Lipid Transfer Protein Sec14p as Target for Antifungal Ergolines.PLoS genetics · 2016Article
- Article
- Characterization of the Sclerotinia sclerotiorum cell wall proteome.Molecular plant pathology · 2016Article
- The role of melanin pathways in extremotolerance and virulence of Fonsecaea revealed by de novo assembly transcriptomics using illumina paired-end sequencing.Studies in mycology · 2016Article
- Copper at the Fungal Pathogen-Host Axis.The Journal of biological chemistry · 2015Review
- Article
- Role of Cln1 during melanization of Cryptococcus neoformans.Frontiers in microbiology · 2015Article
- Article
- Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis.Cold Spring Harbor perspectives in medicine · 2014Review
- Pigment production on L-tryptophan medium by Cryptococcus gattii and Cryptococcus neoformans.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Laccase is a major virulence factor of the pathogenic fungus Cryptococcus neoformans, which afflicts both immunocompetent and immunocompromised individuals. In the present study, laccase was expressed in C. neoformans lac1Delta cells as a fusion protein with an N-terminal green fluorescent protein (GFP) using C. neoformans codon usage. The fusion protein was robustly localized to the cell wall at physiological pH, but it was mislocalized at low pH. Structural analysis of the laccase identified a C-terminal region unique to C. neoformans, and expression studies showed that the region was required for efficient transport to the cell wall both in vitro and during infection of mouse lungs. During infection of mice, adherence to alveolar macrophages was also associated with a partial mislocalization of GFP-laccase within cytosolic vesicles. In addition, recovery of cryptococcal cells from lungs of two strains of mice (CBA/J and Swiss Albino) later in infection was also associated with cytosolic mislocalization, but cells from the brain showed almost exclusive localization to cell walls, suggesting that there was more efficient cell wall targeting during infection of the brain. These data suggest that host cell antifungal defenses may reduce effective cell wall targeting of laccase during infection of the lung but not during infection of the brain, which may contribute to a more predominant role for the enzyme during infection of the brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.