ArticleBritish journal of pharmacology2006
Roles of M2 and M3 muscarinic receptors in cholinergic nerve-induced contractions in mouse ileum studied with receptor knockout mice.
Article in British journal of pharmacology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- Atropine-functionalized gold nanoparticles binding to muscarinic receptors after passage across the intestinal epithelium.Royal Society open science · 2022Article
- A Potential Role of Cholinergic Dysfunction on Impaired Colon Motility in Experimental Intestinal Chagas Disease.Journal of neurogastroenterology and motility · 2022Article
- Functions of Muscarinic Receptor Subtypes in Gastrointestinal Smooth Muscle: A Review of Studies with Receptor-Knockout Mice.International journal of molecular sciences · 2021Review
- Involvement of transient receptor potential melastatin 4 channels in the resting membrane potential setting and cholinergic contractile responses in mouse detrusor and ileal smooth muscles.The Journal of veterinary medical science · 2019Article
- Spasmolytic activity of Aquilariae Lignum Resinatum extract on gastrointestinal motility involves muscarinic receptors, calcium channels and NO release.Pharmaceutical biology · 2018Article
- Possible antagonistic effects of the TRPC4 channel blocker ML204 on MThe Journal of veterinary medical science · 2018Article
- Article
- Alterations of colonic contractility in an interleukin-10 knockout mouse model of inflammatory bowel disease.Journal of neurogastroenterology and motility · 2015Article
- Acupuncture at heterotopic acupoints enhances jejunal motility in constipated and diarrheic rats.World journal of gastroenterology · 2014Article
- Spasmolytic and Anti-Spasmodic Action of Trachyspermum ammi Essence on Rat's Ileum Contraction.North American journal of medical sciences · 2014Article
- Effects of ginsenoside Re on rat jejunal contractility.Journal of natural medicines · 2014Article
- Cholinergic neuromuscular transmission mediated by interstitial cells of Cajal in the myenteric layer in mouse ileal longitudinal smooth muscles.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- SMB myosin heavy chain knockout enhances tonic contraction and reduces the rate of force generation in ileum and stomach antrum.American journal of physiology. Cell physiology · 2013Article
- Alterations of colonic contractility in long-term diabetic rat model.Journal of neurogastroenterology and motility · 2011Article
- Muscarinic activation of Ca2+-activated Cl- current in interstitial cells of Cajal.The Journal of physiology · 2011Article
- Immunoglobulins from scleroderma patients inhibit the muscarinic receptor activation in internal anal sphincter smooth muscle cells.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Multiple muscarinic pathways mediate the suppression of voltage-gated Ca2+ channels in mouse intestinal smooth muscle cells.British journal of pharmacology · 2009Article
- Three distinct muscarinic signalling pathways for cationic channel activation in mouse gut smooth muscle cells.The Journal of physiology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
background and purposeThe functional roles of M(2) and M(3) muscarinic receptors in neurogenic cholinergic contractions in gastrointestinal tracts remain to be elucidated. To address this issue, we studied cholinergic nerve-induced contractions in the ileum using mutant mice lacking M(2) or M(3) receptor subtypes. EXPERIMENTAL APPROACH: Contractile responses to transmural electrical (TE) stimulation were isometrically recorded in ileal segments from M(2)-knockout (KO), M(3)-KO, M(2)/M(3)-double KO, and wild-type mice. KEY
resultsTE stimulation at 2-50 Hz frequency-dependently evoked a fast, brief contraction followed by a slower, longer one in wild-type, M(2)-KO or M(3)-KO mouse preparations. Tetrodotoxin blocked both the initial and later contractions, while atropine only inhibited the initial contractions. The initial cholinergic contractions were significantly greater in wild-type than M(2)-KO or M(3)-KO mice; the respective mean amplitudes at 50 Hz were 91, 74 and 68 % of 70mM K(+)-induced contraction. Pretreatment with pertussis toxin blocked the cholinergic contractions in M(3)-KO but not in M(2)-KO mice. Cholinergic contractions also remained in wild-type preparations, but their sizes were reduced by 20-30 % at 10-50 Hz. In M(2)/M(3)-double KO mice, TE stimulation evoked only slow, noncholinergic contractions, which were significantly greater in sizes than in any of the other three mouse strains. CONCLUSION AND IMPLICATIONS: These results demonstrate that M(2) and M(3) receptors participate in mediating cholinergic contractions in mouse ileum with the latter receptors assuming a greater role. Our data also suggest that the lack of both M(2) and M(3) receptors causes upregulation of noncholinergic excitatory innervation of the gut smooth muscle.
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