ArticleEukaryotic cell2007
Cryptococcal lipid metabolism: phospholipase B1 is implicated in transcellular metabolism of macrophage-derived lipids.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
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- Comparative Genomics Reveals Genetic Adaptations to Diving-Associated Foraging in Anseriformes.Ecology and evolution · 2026Article
- Reconsidering the blood-brain barrier: histopathology and microanatomy of cryptococcal CNS infection.Microbiology and molecular biology reviews : MMBR · 2025Review
- Pathogenicity and virulence of Cryptococcus neoformans from an environmental perspective.Virulence · 2025Review
- Reveal key genes and factors affecting athletes performance in endurance sports using bioinformatic technologies.BMC genomic data · 2023Article
- Transcriptional Profiles Elucidate Differential Host Responses to Infection withJournal of fungi (Basel, Switzerland) · 2022Article
- Influence of Pathogen Carbon Metabolism on Interactions With Host Immunity.Frontiers in cellular and infection microbiology · 2022Review
- Treatment strategies for cryptococcal infection: challenges, advances and future outlook.Nature reviews. Microbiology · 2021Review
- Mechanisms of fungal dissemination.Cellular and molecular life sciences : CMLS · 2021Review
- Dangerous Liaisons: Interactions ofPathogens (Basel, Switzerland) · 2020Review
- How Environmental Fungi Cause a Range of Clinical Outcomes in Susceptible Hosts.Journal of molecular biology · 2019Review
- Lipids Affect the Cryptococcus neoformans-Macrophage Interaction and Promote Nonlytic Exocytosis.Infection and immunity · 2017Article
- Virulence-Associated Enzymes of Cryptococcus neoformans.Eukaryotic cell · 2015Review
- Cryptococcus neoformans and Cryptococcus gattii, the etiologic agents of cryptococcosis.Cold Spring Harbor perspectives in medicine · 2014Review
- Phospholipase B is activated in response to sterol removal and stimulates acrosome exocytosis in murine sperm.The Journal of biological chemistry · 2013Article
- Lipolytic enzymes involved in the virulence of human pathogenic fungi.Mycobiology · 2013Article
- Peroxisomal and mitochondrial β-oxidation pathways influence the virulence of the pathogenic fungus Cryptococcus neoformans.Eukaryotic cell · 2012Article
- Paradoxical roles of alveolar macrophages in the host response to Cryptococcus neoformans.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2012Review
- Adaptation of Cryptococcus neoformans to mammalian hosts: integrated regulation of metabolism and virulence.Eukaryotic cell · 2012Review
- SEC14 is a specific requirement for secretion of phospholipase B1 and pathogenicity of Cryptococcus neoformans.Molecular microbiology · 2011Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryptococci survive and replicate within macrophages and can use exogenous arachidonic acid for the production of eicosanoids. Phospholipase B1 (PLB1) has a putative, but uninvestigated, role in these processes. We have shown that uptake and esterification of radiolabeled arachidonic, palmitic, and oleic acids by the Cryptococcus neoformans var. grubii H99 wild-type strain and its PLB1 deletion mutant strain (the Deltaplb1 strain) are independent of PLB1, except under hyperosmolar stress. Similarly, PLB1 was required for metabolism of 1-palmitoyl lysophosphatidylcholine (LysoPC), which is toxic to eukaryotic cell membranes, under hyperosmolar conditions. During both logarithmic and stationary phases of growth, the physiologically relevant phospholipids, dipalmitoyl phosphatidylcholine (DPPC) and dioleoyl phosphatidylcholine, were taken up and metabolized via PLB1. Exogenous DPPC did not enhance growth in the presence of glucose as a carbon source but could support it for at least 24 h in glucose-free medium. Detoxification of LysoPC by reacylation occurred in both the H99 wild-type and the Deltaplb1 strains in the presence of glucose, but PLB1 was required when LysoPC was the sole carbon source. This indicates that both energy-independent (via PLB1) and energy-dependent transacylation pathways are active in cryptococci. Phospholipase A(1) activity was identified by PLB1-independent degradation of 1-palmitoyl-2-arachidonoyl phosphatidylcholine, but the arachidonoyl LysoPC formed was not detoxified by reacylation. Using the human macrophage-like cell line THP-1, we demonstrated the PLB1-dependent incorporation of macrophage-derived arachidonic acid into cryptococcal lipids during cryptococcus-phagocyte interaction. This pool of arachidonate can be sequestered for eicosanoid production by the fungus and/or suppression of host phagocytic activity, thus diminishing the immune response.
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