ArticleJournal of bacteriology2007
Streptococcus iniae capsule impairs phagocytic clearance and contributes to virulence in fish.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 93 citations in OpenAlex.
- Proteomic and Functional Analysis Reveals Temperature-Driven Immune Evasion Strategies ofBiology · 2025Article
- Determinants of bacterial survival and proliferation in blood.FEMS microbiology reviews · 2024Review
- Expansion of healthcare-associated hypervirulent KPC-2-producingOne health (Amsterdam, Netherlands) · 2023Article
- Detoxification of reactive oxygen species by the hyaluronic acid capsule of group AInfection and immunity · 2023Article
- Whole Genomic Characterization ofVaccines · 2023Article
- A Novel Conserved Protein in Streptococcus agalactiae, BvaP, Is Important for Vaginal Colonization and Biofilm Formation.mSphere · 2022Article
- Streptococcosis a Re-Emerging Disease in Aquaculture: Significance and Phytotherapy.Animals : an open access journal from MDPI · 2022Review
- Review
- CRISPR Contributes to Adhesion, Invasion, and Biofilm Formation in Streptococcus agalactiae by Repressing Capsular Polysaccharide Production.Microbiology spectrum · 2022Article
- The Antibacterial Activity of Thymol Against Drug-ResistantFrontiers in microbiology · 2022Article
- Streptococcal Infections in Marine Mammals.Microorganisms · 2021Review
- Functional Analysis of Two NovelMicroorganisms · 2020Article
- Review on Immersion Vaccines for Fish: An Update 2019.Microorganisms · 2019Review
- Microevolution of Streptococcus agalactiae ST-261 from Australia Indicates Dissemination via Imported Tilapia and Ongoing Adaptation to Marine Hosts or Environment.Applied and environmental microbiology · 2018Article
- Neutrophil derived LTB4 induces macrophage aggregation in response to encapsulated Streptococcus iniae infection.PloS one · 2017Article
- Cloning and Characterization of Surface-Localized α-Enolase of Streptococcus iniae, an Effective Protective Antigen in Mice.International journal of molecular sciences · 2015Article
- Article
- Streptococcus iniae SF1: complete genome sequence, proteomic profile, and immunoprotective antigens.PloS one · 2014Article
- Reductive evolution in Streptococcus agalactiae and the emergence of a host adapted lineage.BMC genomics · 2013Article
- Innate immune response to Streptococcus iniae infection in zebrafish larvae.Infection and immunity · 2013Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surface capsular polysaccharides play a critical role in protecting several pathogenic microbes against innate host defenses during infection. Little is known about virulence mechanisms of the fish pathogen Streptococcus iniae, though indirect evidence suggests that capsule could represent an important factor. The putative S. iniae capsule operon contains a homologue of the cpsD gene, which is required for capsule polymerization and export in group B Streptococcus and Streptococcus pneumoniae. To elucidate the role of capsule in the S. iniae infectious process, we deleted cpsD from the genomes of two virulent S. iniae strains by allelic exchange mutagenesis to generate the isogenic capsule-deficient DeltacpsD strains. Compared to wild-type S. iniae, the DeltacpsD mutants had a predicted reduction in buoyancy and cell surface negative charge. Transmission electron microscopy confirmed a decrease in the abundance of extracellular capsular polysaccharide. Gas-liquid chromatography-mass spectrometry analysis of the S. iniae extracellular polysaccharides showed the presence of l-fucose, d-mannose, d-galactose, d-glucose, d-glucuronic acid, N-acetyl-d-galactosamine, and N-acetyl-d-glucosamine, and all except mannose were reduced in concentration in the isogenic mutant. The DeltacpsD mutants were highly attenuated in vivo in a hybrid striped bass infection challenge despite being more adherent and invasive to fish epithelial cells and more resistant to cationic antimicrobial peptides than wild-type S. iniae. Increased susceptibility of the S. iniae DeltacpsD mutants to phagocytic killing in whole fish blood and by a fish macrophage cell line confirmed the role of capsule in virulence and highlighted its antiphagocytic function. In summary, we report a genetically defined study on the role of capsule in S. iniae virulence and provide preliminary analysis of S. iniae capsular polysaccharide sugar components.
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