ArticleBiophysical journal2007
VASP governs actin dynamics by modulating filament anchoring.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 64 citations in OpenAlex.
- On multistability and constitutive relations of cell motion on fibronectin lanes.Biophysical journal · 2023Article
- Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Cytoskeleton Force Exertion in Bulk Cytoplasm.Frontiers in cell and developmental biology · 2020Review
- VASP Regulates NK Cell Lytic Granule Convergence.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Cell-cell adhesion interface: orthogonal and parallel forces from contraction, protrusion, and retraction.F1000Research · 2018Review
- Toward the reconstitution of synthetic cell motility.Cell adhesion & migration · 2016Review
- On-Chip Quantitative Measurement of Mechanical Stresses During Cell Migration with Emulsion Droplets.Scientific reports · 2016Article
- A toolbox to explore the mechanics of living embryonic tissues.Seminars in cell & developmental biology · 2016Review
- Reconstitution of actin-based motility by vasodilator-stimulated phosphoprotein (VASP) depends on the recruitment of F-actin seeds from the solution produced by cofilin.The Journal of biological chemistry · 2014Article
- Quantifying cell-generated mechanical forces within living embryonic tissues.Nature methods · 2014Article
- Formation of transient lamellipodia.PloS one · 2014Article
- Cell-sized liposomes reveal how actomyosin cortical tension drives shape change.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Multiple actin binding domains of Ena/VASP proteins determine actin network stiffening.European biophysics journal : EBJ · 2012Article
- Cytoskeletal modulation of lipid interactions regulates Lck kinase activity.The Journal of biological chemistry · 2012Article
- Actin network growth under load.Biophysical journal · 2012Article
- Choosing orientation: influence of cargo geometry and ActA polarization on actin comet tails.Molecular biology of the cell · 2012Article
- Turnover of branched actin filament networks by stochastic fragmentation with ADF/cofilin.Molecular biology of the cell · 2011Article
- Observation and kinematic description of long actin tracks induced by spherical beads.Biophysical journal · 2010Article
- Effects of molecular-scale processes on observable growth properties of actin networks.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- Models for actin polymerization motors.Journal of mathematical biology · 2009Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin filament dynamics at the cell membrane are important for cell-matrix and cell-cell adhesions and the protrusion of the leading edge. Since actin filaments must be connected to the cell membrane to exert forces but must also detach from the membrane to allow it to move and evolve, the balance between actin filament tethering and detachment at adhesion sites and the leading edge is key for cell shape changes and motility. How this fine tuning is performed in cells remains an open question, but possible candidates are the Drosophila enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) family of proteins, which localize to dynamic actin structures in the cell. Here we study VASP-mediated actin-related proteins 2/3 (Arp2/3) complex-dependent actin dynamics using a substrate that mimics the fluid properties of the cell membrane: an oil-water interface. We show evidence that polymerization activators undergo diffusion and convection on the fluid surface, due to continual attachment and detachment to the actin network. These dynamics are enhanced in the presence of VASP, and we observe cycles of catastrophic detachment of the actin network from the surface, resulting in stop-and-go motion. These results point to a role for VASP in the modulation of filament anchoring, with implications for actin dynamics at cell adhesions and at the leading edge of the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.