ArticleGenomics2007
BAC clones generated from sheared DNA.
Article in Genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Variation and Evolution of Human Centromeres: A Field Guide and Perspective.Annual review of genetics · 2021Review
- Expanding studies of chromosome structure and function in the era of T2T genomics.Human molecular genetics · 2021Review
- Construction of JRG (Japanese reference genome) with single-molecule real-time sequencing.Human genome variation · 2019Article
- Single-molecule measurements of viral ssRNA packaging.RNA (New York, N.Y.) · 2017Article
- The AKR gene family and modifying sex ratios in palms through abiotic stress responsiveness.Functional & integrative genomics · 2015Article
- The organization and evolution of the Responder satellite in species of the Drosophila melanogaster group: dynamic evolution of a target of meiotic drive.BMC evolutionary biology · 2014Article
- Characterization of rubber tree microRNA in phytohormone response using large genomic DNA libraries, promoter sequence and gene expression analysis.Molecular genetics and genomics : MGG · 2014Article
- The genome sequence of the fungal pathogen Fusarium virguliforme that causes sudden death syndrome in soybean.PloS one · 2014Article
- Illumina TruSeq synthetic long-reads empower de novo assembly and resolve complex, highly-repetitive transposable elements.PloS one · 2014Article
- Exploring the genome of the salt-marsh Spartina maritima (Poaceae, Chloridoideae) through BAC end sequence analysis.Plant molecular biology · 2013Article
- The origin, global distribution, and functional impact of the human 8p23 inversion polymorphism.Genome research · 2012Article
- Article
- A hybrid BAC physical map of potato: a framework for sequencing a heterozygous genome.BMC genomics · 2011Article
- A pair of new BAC and BIBAC vectors that facilitate BAC/BIBAC library construction and intact large genomic DNA insert exchange.Plant methods · 2011Article
- The 19 genomes of Drosophila: a BAC library resource for genus-wide and genome-scale comparative evolutionary research.Genetics · 2011Article
- Genome-wide BAC-end sequencing of Cucumis melo using two BAC libraries.BMC genomics · 2010Article
- Characterization of a deep-coverage carrot (Daucus carota L.) BAC library and initial analysis of BAC-end sequences.Molecular genetics and genomics : MGG · 2009Article
- Drosophila telomeric retrotransposons derived from an ancestral element that was recruited to replace telomerase.Genome research · 2007Article
- Islands of euchromatin-like sequence and expressed polymorphic sequences within the short arm of human chromosome 21.Genome research · 2007Article
- End-sequencing and characterization of silkworm (Bombyx mori) bacterial artificial chromosome libraries.BMC genomics · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
BAC libraries generated from restriction-digested genomic DNA display representational bias and lack some sequences. To facilitate completion of genome projects, procedures have been developed to create BACs from DNA physically sheared to create fragments extending up to 200 kb. The DNA fragments were repaired to create blunt ends and ligated to a new BAC vector. This approach has been tested by generating BAC libraries from Drosophila DNA with insert lengths between 50 and 150 kb. The libraries lack chimeric clone problems as determined by mapping paired BAC-end sequences to the assembled fly genome sequence. The utility of "sheared" libraries was demonstrated by closure of a previous clone gap and by isolation of clones from telomeric regions, which were notably absent from previous Drosophila BAC libraries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.