Evidence map›Paper›PMID 17097606›Full record

ArticleBiochemical and biophysical research communications2006

Opioid receptor-independent protection of ischemic rat hepatocytes by morphine.

Jae-Sung Kim, John J Lemasters

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Jae-Sung KimDepartment of Surgery, University of Florida, Gainesville, FL 32610, USA.
John J Lemasters
Medical University of South Carolina · USUniversity of Florida · US

Funding

PILOT AND FEASIBILITY STUDIESP30DK034987 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ROBERT S. SANDLER · 1985 to 2026
$30.5M
TNF(ALPHA)- AND FAS-INDUCED HEPATIC APOPTOSISP01DK059340 · NIDDK · UNIVERSITY OF NORTH CAROLINA CHAPEL HILL · PI LEMASTERS, JOHN J · 2001 to 2005
$4.7M
LIVER PRESERVATION FOR TRANSPLANTATIONR37DK037034 · NIDDK · UNIVERSITY OF NORTH CAROLINA CHAPEL HILL · PI LEMASTERS, JOHN J · 1998 to 2007
$4.0M
NIDDK NIH HHS P01 DK059340NIDDK NIH HHS P30 DK034987NIDDK NIH HHS R37 DK037034
6 · The paper itself

Abstract

We studied the role of morphine in anoxia/reoxygenation injury to hepatocytes. Overnight cultured rat hepatocytes were incubated in anoxic buffer at pH 6.2 for 4h and reoxygenated at pH 7.4 for 2h to simulate anoxia/reoxygenation. Some hepatocytes were preincubated with 50 microM morphine for 10 min prior to onset of anoxia/reoxygenation. To study the effect of morphine on nitric oxide (NO), hepatocytes were loaded with 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM). Changes in NO concentration were assessed with a multi-well fluorescence reader and confocal microscopy. Morphine substantially improved cell viability after reoxygenation and increased NO generation, which was blocked by ATP-sensitive potassium channel blockers. Confocal images revealed that the increase in NO occurred mainly at the cytosol. However, treatment with opioid receptor antagonists did not reverse cytoprotection by morphine. These results indicate that morphine prevents anoxia/reoxygenation injury to hepatocytes. Protective mechanisms are associated with the potassium channels and NO, but are independent of opioid receptor-mediated signaling.

Indexed as

CytoprotectionAnimalsCells, CulturedFluoresceinsHepatocytesHypoxiaIschemiaMicroscopy, ConfocalMorphineNitric OxideOxygenPotassium Channel BlockersPotassium ChannelsRatsReceptors, Opioid4-amino-5-methylamino-2',7'-difluorofluorescein diacetateFluoresceinsMorphineNitric OxideOxygenPotassium Channel BlockersPotassium ChannelsReceptors, Opioid

Identifiers

PMID17097606
PMCPMC1783610
OpenAlexW2074759996

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.