ArticleMolecular and biochemical parasitology2007
Characterization of protein kinase CK2 from Trypanosoma brucei.
Article in Molecular and biochemical parasitology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 62 citations in OpenAlex.
- An essentialFrontiers in parasitology · 2023Article
- Article
- Chromatin-Associated Protein Complexes Link DNA Base J and Transcription Termination inmSphere · 2021Article
- CTS tag-based methods for investigating mitochondrial RNA modification factors in Trypanosoma brucei.Methods in enzymology · 2021Article
- Poly(A) binding KPAF4/5 complex stabilizes kinetoplast mRNAs in Trypanosoma brucei.Nucleic acids research · 2020Article
- Article
- Pentatricopeptide repeat poly(A) binding protein KPAF4 stabilizes mitochondrial mRNAs in Trypanosoma brucei.Nature communications · 2019Article
- PPR polyadenylation factor defines mitochondrial mRNA identity and stability in trypanosomes.The EMBO journal · 2017Article
- RNAi screening identifies Trypanosoma brucei stress response protein kinases required for survival in the mouse.Scientific reports · 2017Article
- RNA Editing TUTase 1: structural foundation of substrate recognition, complex interactions and drug targeting.Nucleic acids research · 2016Article
- Investigating RNA editing factors from trypanosome mitochondria.Methods (San Diego, Calif.) · 2016Article
- The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr.RNA (New York, N.Y.) · 2016Article
- Ribosome-associated pentatricopeptide repeat proteins function as translational activators in mitochondria of trypanosomes.Molecular microbiology · 2016Article
- A tRNA methyltransferase paralog is important for ribosome stability and cell division in Trypanosoma brucei.Scientific reports · 2016Article
- Antisense Transcripts Delimit Exonucleolytic Activity of the Mitochondrial 3' Processome to Generate Guide RNAs.Molecular cell · 2016Article
- Bloodstream form Trypanosoma brucei do not require mRPN1 for gRNA processing.RNA (New York, N.Y.) · 2015Article
- RNA binding and core complexes constitute the U-insertion/deletion editosome.Molecular and cellular biology · 2014Article
- Ribosome biogenesis requires a highly diverged XRN family 5'->3' exoribonuclease for rRNA processing in Trypanosoma brucei.RNA (New York, N.Y.) · 2013Article
- Trypanosoma brucei Tb927.2.6100 is an essential protein associated with kinetoplast DNA.Eukaryotic cell · 2013Article
- The T. brucei TRM5 methyltransferase plays an essential role in mitochondrial protein synthesis and function.RNA (New York, N.Y.) · 2013Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
CK2 is a ubiquitous but enigmatic kinase. The difficulty in assigning a role to CK2 centers on the fact that, to date, no biologically relevant modulator of its function has been identified. One common theme revolves around a constellation of known substrates involved in growth control, compatible with its concentration in the nucleus and nucleolus. We had previously described the identification of two catalytic subunits of CK2 in Trypanosoma brucei and characterized one of them. Here we report the characterization of the second catalytic subunit, CK2alpha', and the identification and characterization of the regulatory subunit CK2beta. All three subunits are primarily localized to the nucleolus in T. brucei. We also show that CK2beta interacts with the nucleolar protein NOG1, adding to the interaction map which previously linked CK2alpha to the nucleolar protein NOPP44/46, which in turn associates with the rRNA binding protein p37. CK2 activity has four distinctive features: near equal affinity for GTP and ATP, heparin sensitivity, and stimulation by polyamines and polybasic peptides. Sequence comparison shows that the parasite orthologues have mutations in residues previously mapped as important in specifying affinity for GTP and stimulation by both polyamines and polybasic peptides. Studies of the enzymatic activity of the T. brucei CK2s show that both the affinity for GTP and stimulation by polyamines have been lost and only the features of heparin inhibition and stimulation by polybasic peptides are conserved.
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