Evidence map›Paper›PMID 17096139›Full record

ArticleParasitology research2007

PCR amplification and sequence analyses of ITS-1 rDNA from Cryptosporidium andersoni in dairy cattle.

Rongqiong Zhou, Guoqing Li, Shumin Xiao, Yanxun Xia, Yuanzhong Guo

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Article in Parasitology research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rongqiong ZhouDepartment of Veterinary Parasitology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Guoqing Li
Shumin Xiao
Yanxun Xia
Yuanzhong Guo

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The internal transcribed spacer 1 (ITS-1) of the ribosomal DNA (rDNA) from GD, HN, and AH strains of Cryptosporidium andersoni was amplified and sequenced to assess whether the ITS-1 rDNA could be used as genetic markers of C. andersoni from different geographic origins in China and to differentiate C. andersoni from other Cryptosporidium species. The result showed that the ITS-1 sequences of GD, HN, and AH strains were basically identical, which were unequivocally different with the sequences of the Cryptosporidium muris and Cryptosporidium parvum registered in the GenBank; however, the ITS-1 sequence of the AH strain differed at three bases compared with that of the other two strains. Our study indicates that the ITS-1 sequences provide useful genetic markers for the identification and differentiation of C. andersoni species and also lay down the foundation for diagnostics of cryptosporidiosis.

Indexed as

AnimalsBase SequenceCattleCattle DiseasesChinaCryptosporidiosisCryptosporidiumDNA, ProtozoanDNA, Ribosomal SpacerMolecular Sequence DataPolymerase Chain ReactionSequence Analysis, DNADNA, ProtozoanDNA, Ribosomal Spacer

Identifiers

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.