ArticleRNA (New York, N.Y.)2007
Cap- and initiator tRNA-dependent initiation of TYMV polyprotein synthesis by ribosomes: evaluation of the Trojan horse model for TYMV RNA translation.
Article in RNA (New York, N.Y.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Host-like RNA Elements Regulate Virus Translation.Viruses · 2024Review
- Non-Canonical Translation Initiation Mechanisms Employed by Eukaryotic Viral mRNAs.Biochemistry. Biokhimiia · 2021Review
- Structural diversity and phylogenetic distribution of valyl tRNA-like structures in viruses.RNA (New York, N.Y.) · 2021Article
- Viral RNA structure-based strategies to manipulate translation.Nature reviews. Microbiology · 2019Review
- Non-canonical Translation in Plant RNA Viruses.Frontiers in plant science · 2017Review
- Viral tRNA Mimicry from a Biocommunicative Perspective.Frontiers in microbiology · 2017Review
- A highly divergent isolate of tomato blistering mosaic virus from Solanum violaefolium.Virus genes · 2016Article
- Messenger RNAs bearing tRNA-like features exemplified by interferon alfa 5 mRNA.Cellular and molecular life sciences : CMLS · 2015Article
- Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection.The Plant journal : for cell and molecular biology · 2012Article
- Multi-domain packing in the aminoacylatable 3' end of a plant viral RNA.Journal of molecular biology · 2010Article
- Role of tRNA-like structures in controlling plant virus replication.Virus research · 2009Review
- Comparison and functional implications of the 3D architectures of viral tRNA-like structures.RNA (New York, N.Y.) · 2009Article
- Virus versus host cell translation love and hate stories.Advances in virus research · 2009Review
- Viral RNA pseudoknots: versatile motifs in gene expression and replication.Nature reviews. Microbiology · 2007Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Turnip yellow mosaic virus (TYMV) RNA directs the translation of two overlapping open reading frames. Competing models have been previously published to explain ribosome access to the downstream polyprotein cistron. The Trojan horse model, based on cell-free experiments, proposes noncanonical cap-independent initiation in which the 3'-terminal tRNA-like structure (TLS) functionally replaces initiator tRNA, and the valine bound to the TLS becomes cis-incorporated into viral protein. The initiation coupling model, based on in vivo expression and ribosome toe-printing studies, proposes a variation of canonical leaky scanning. Here, we have re-examined the wheat germ extract experiments that led to the Trojan horse model, incorporating a variety of controls. We report that (1) translation in vitro from the polyprotein AUG of TYMV RNA is unchanged after removal of the 3' TLS but is stimulated by the presence of a 5'-cap; (2) the presence of free cap analog or edeine (which interferes with initiation at the ribosomal P site and its tRNA(i) (Met) involvement) inhibits translation from the polyprotein AUG; (3) the toe-prints of immediately post-initiation ribosomes on TYMV RNA are similar with and without an intact TLS; and (4) significant deacylation of valyl-TYMV RNA in wheat germ extract can complicate the detection of cis-incorporation. These results favor the initiation coupling model.
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