ArticleArchives of biochemistry and biophysics2006
Traction forces of fibroblasts are regulated by the Rho-dependent kinase but not by the myosin light chain kinase.
Article in Archives of biochemistry and biophysics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 125 citations in OpenAlex.
- Measurement of cellular traction forces during confined migration.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Myosin-independent stiffness sensing by fibroblasts is regulated by the viscoelasticity of flowing actin.Communications materials · 2024Article
- SFAlab: image-based quantification of mechano-active ventral actin stress fibers in adherent cells.Frontiers in cell and developmental biology · 2023Article
- Mechanical force drives the initial mesenchymal-epithelial interaction during skin organoid development.Theranostics · 2023Article
- Wound Healing from an Actin Cytoskeletal Perspective.Cold Spring Harbor perspectives in biology · 2022Review
- Actomyosin contractility requirements and reciprocal cell-tissue mechanics for cancer cell invasion through collagen-based channels.The European physical journal. E, Soft matter · 2022Article
- Condensation tendency and planar isotropic actin gradient induce radial alignment in confined monolayers.eLife · 2021Article
- The Crossroads between RAS and RHO Signaling Pathways in Cellular Transformation, Motility and Contraction.Genes · 2021Review
- On the adhesion-velocity relation and length adaptation of motile cells on stepped fibronectin lanes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds.Science advances · 2020Article
- Challenging human somatic testicular cell reassembly by protein kinase inhibition -setting up a functional in vitro test system.Scientific reports · 2020Article
- Hemidesmosomes modulate force generation via focal adhesions.The Journal of cell biology · 2020Article
- Cell-mediated matrix stiffening accompanies capillary morphogenesis in ultra-soft amorphous hydrogels.Biomaterials · 2020Article
- Probing Single-Cell Mechanical Allostasis Using Ultrasound Tweezers.Cellular and molecular bioengineering · 2019Article
- Data on the negative regulation of invadopodia activity by MLCK.Data in brief · 2019Article
- A systems mechanobiology model to predict cardiac reprogramming outcomes on different biomaterials.Biomaterials · 2018Article
- Mechanoreciprocity in cell migration.Nature cell biology · 2018Review
- Comparative muscle proteomics/phosphoproteomics analysis provides new insight for the biosafety evaluation of fat-1 transgenic cattle.Transgenic research · 2017Article
- Emergent cellular self-organization and mechanosensation initiate follicle pattern in the avian skin.Science (New York, N.Y.) · 2017Article
- Microtubules acquire resistance from mechanical breakage through intralumenal acetylation.Science (New York, N.Y.) · 2017Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Adhesive cells show complex mechanical interactions with the substrate, however the exact mechanism of such interactions, termed traction forces, is still unclear. To address this question we have measured traction forces of fibroblasts treated with agents that affect the myosin II-dependent contractile mechanism. Using the potent myosin II inhibitor blebbistatin, we demonstrate that traction forces are strongly dependent on a functional myosin II heavy chain. Since myosin II is regulated by both the myosin light chain kinase (MLCK) and, directly or indirectly, the Rho-associated kinase (ROCK), we examined the effects of inhibitors against these kinases. Interestingly, inhibition of the myosin light chain kinase had no detectable effect, while inhibition of the Rho-dependent kinase caused strong inhibition of traction forces. Our results indicate that ROCK and MLCK play non-redundant roles in regulating myosin II functions, and that a subset of myosin II, regulated by the Rho small GTPase, may be responsible for the regulation of traction forces in migrating fibroblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.