ArticleMolecular biology reports2007
Subtractive differential display: a modified differential display technique for isolating differentially expressed genes.
Article in Molecular biology reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Isolation and analysis of genes mainly expressed in adult mouse heart using subtractive hybridization cDNA library.Molecular biology reports · 2012Article
- Gene expression profiling in zebrafish embryos exposed to diclofenac, an environmental toxicant.Molecular biology reports · 2012Article
- Supplementation of deprenyl attenuates age associated alterations in rat cerebellum.Molecular biology reports · 2010Article
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Authors and funding
2 authors.
Funding
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Abstract
Differential display (DD) is a novel PCR-based technique, very commonly used to study differentially expressed genes at the mRNA level. In this paper we report a modified version of this technique that we have used to study the differences between the mRNA population from brain tissue of adult and old rats. We have modified the technique to enhance reproducibility and reduce false positives and redundancy. It is fast and does not require any expensive or uncommon reagent. We choose to call it as subtractive differential display as it is a differential display performed over subtracted mRNA population. We have used this protocol successfully to clone a number of age-related differentially expressed sequences from rat brain that need to be sequenced to establish the gene identity.
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Registered trials
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