ArticleMolecular biology of the cell2007
High-content microscopy identifies new neurite outgrowth regulators.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
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- Speckle-structured illumination for 3D phase and fluorescence computational microscopy.Biomedical optics express · 2019Article
- Morphometric and computational assessments to evaluate neuron survival and maturation within compartmentalized microfluidic devices: The influence of design variation on diffusion-driven nutrient transport.Neuroscience letters · 2019Article
- Computational structured illumination for high-content fluorescence and phase microscopy.Biomedical optics express · 2019Article
- Microtubule plus-end tracking proteins in neuronal development.Cellular and molecular life sciences : CMLS · 2016Review
- Botanical drug puerarin coordinates with nerve growth factor in the regulation of neuronal survival and neuritogenesis via activating ERK1/2 and PI3K/Akt signaling pathways in the neurite extension process.CNS neuroscience & therapeutics · 2015Article
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- The NAP motif of activity-dependent neuroprotective protein (ADNP) regulates dendritic spines through microtubule end binding proteins.Molecular psychiatry · 2014Article
- Transcriptional analysis of apoptotic cerebellar granule neurons following rescue by gastric inhibitory polypeptide.International journal of molecular sciences · 2014Article
- The kinesin-2 family member KIF3C regulates microtubule dynamics and is required for axon growth and regeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Claudins reign: The claudin/EMP/PMP22/γ channel protein family in C. elegans.Tissue barriers · 2013Review
- TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation.American journal of physiology. Cell physiology · 2011Article
- TGF-β signaling is required for maintenance of retinal ganglion cell differentiation and survival.Neuroscience · 2011Article
- Chemical priming for spinal cord injury: a review of the literature part II-potential therapeutics.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2011Review
- Chemical priming for spinal cord injury: a review of the literature. Part I-factors involved.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2011Review
- NeurphologyJ: an automatic neuronal morphology quantification method and its application in pharmacological discovery.BMC bioinformatics · 2011Article
- A rapid, inexpensive high throughput screen method for neurite outgrowth.Current chemical genomics · 2010Article
- Differential effects of myocilin and optineurin, two glaucoma genes, on neurite outgrowth.The American journal of pathology · 2010Article
- In-tube transfection improves the efficiency of gene transfer in primary neuronal cultures.Journal of neuroscience methods · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurons, with their long axons and elaborate dendritic arbour, establish the complex circuitry that is essential for the proper functioning of the nervous system. Whereas a catalogue of structural, molecular, and functional differences between axons and dendrites is accumulating, the mechanisms involved in early events of neuronal differentiation, such as neurite initiation and elongation, are less well understood, mainly because the key molecules involved remain elusive. Here we describe the establishment and application of a microscopy-based approach designed to identify novel proteins involved in neurite initiation and/or elongation. We identified 21 proteins that affected neurite outgrowth when ectopically expressed in cells. Complementary time-lapse microscopy allowed us to discriminate between early and late effector proteins. Localization experiments with GFP-tagged proteins in fixed and living cells revealed a further 14 proteins that associated with neurite tips either early or late during neurite outgrowth. Coexpression experiments of the new effector proteins provide a first glimpse on a possible functional relationship of these proteins during neurite outgrowth. Altogether, we demonstrate the potential of the systematic microscope-based screening approaches described here to tackle the complex biological process of neurite outgrowth regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.