ArticleMolecular biology of the cell2007
The interplay of the N- and C-terminal domains of MCAK control microtubule depolymerization activity and spindle assembly.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- KIF2C is essential for meiosis and manchette dynamics in male mice.Frontiers in cell and developmental biology · 2025Article
- Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives.Molecular and cellular biochemistry · 2024Review
- KIF2C affects sperm cell differentiation in patients with Klinefelter syndrome, as revealed by RNA-Seq and scRNA-Seq data.FEBS open bio · 2022Article
- Article
- Ternary complex of Kif2A-bound tandem tubulin heterodimers represents a kinesin-13-mediated microtubule depolymerization reaction intermediate.Nature communications · 2018Article
- Kif2 localizes to a subdomain of cortical endoplasmic reticulum that drives asymmetric spindle position.Nature communications · 2017Article
- Article
- The family-specific α4-helix of the kinesin-13, MCAK, is critical to microtubule end recognition.Open biology · 2016Article
- The far C-terminus of MCAK regulates its conformation and spindle pole focusing.Molecular biology of the cell · 2016Article
- Expression and characterization of three Aurora kinase C splice variants found in human oocytes.Molecular human reproduction · 2015Article
- Article
- Nucleotide exchange in dimeric MCAK induces longitudinal and lateral stress at microtubule ends to support depolymerization.Structure (London, England : 1993) · 2014Article
- Aurora A orchestrates entosis by regulating a dynamic MCAK-TIP150 interaction.Journal of molecular cell biology · 2014Article
- Polo-like kinase 1 regulates the stability of the mitotic centromere-associated kinesin in mitosis.Oncotarget · 2014Article
- Aurora B inhibits MCAK activity through a phosphoconformational switch that reduces microtubule association.Current biology : CB · 2013Article
- Intrinsic Disorder in the Kinesin Superfamily.Biophysical reviews · 2013Article
- Kinesin-13 and tubulin posttranslational modifications regulate microtubule growth in axon regeneration.Developmental cell · 2012Article
- Control of MCAK degradation and removal from centromeres.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Phosphatidylinositol 4-phosphate 5-kinase alpha (PIPKα) regulates neuronal microtubule depolymerase kinesin, KIF2A and suppresses elongation of axon branches.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The kinesin-13 MCAK has an unconventional ATPase cycle adapted for microtubule depolymerization.The EMBO journal · 2011Article
Corrections and comments
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5 authors.
Funding
Abstract
Spindle assembly and accurate chromosome segregation require the proper regulation of microtubule dynamics. MCAK, a Kinesin-13, catalytically depolymerizes microtubules, regulates physiological microtubule dynamics, and is the major catastrophe factor in egg extracts. Purified GFP-tagged MCAK domain mutants were assayed to address how the different MCAK domains contribute to in vitro microtubule depolymerization activity and physiological spindle assembly activity in egg extracts. Our biochemical results demonstrate that both the neck and the C-terminal domain are necessary for robust in vitro microtubule depolymerization activity. In particular, the neck is essential for microtubule end binding, and the C-terminal domain is essential for tight microtubule binding in the presence of excess tubulin heterodimer. Our physiological results illustrate that the N-terminal domain is essential for regulating microtubule dynamics, stimulating spindle bipolarity, and kinetochore targeting; whereas the C-terminal domain is necessary for robust microtubule depolymerization activity, limiting spindle bipolarity, and enhancing kinetochore targeting. Unexpectedly, robust MCAK microtubule (MT) depolymerization activity is not needed for sperm-induced spindle assembly. However, high activity is necessary for proper physiological MT dynamics as assayed by Ran-induced aster assembly. We propose that MCAK activity is spatially controlled by an interplay between the N- and C-terminal domains during spindle assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.